Invertase inhibitor was extracted from potato tubers and purified nearly 1000-fold. The purification procedure involved precipitation at pH 4.0, fractionation with ammonium sulfate, adsorption on alumina Cgamma gel, and gel filtration on Sephadex G-100 and DEAE-Sephadex A-50. The product obtained was homogeneous to electrophoresis on polyacrylamide gel. Exclusion chromatography on Sephadex G-10...

Abstract Soil microorganisms are closely related to soil environmental factors. To explore the effects of lime nitrogen, nitrogen combined with biocontrol agents on factors and fungi their correlation in tobacco fields, four treatments were set: 1_1: control group; 1_2: 1_3: 1_4: group. samples taken during growth periods plants, analyzed by high-throughput sequencing. The results showed that b...

Vacuolar invertase is one of the key enzymes in sucrose metabolism that irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose in plants. In this research, three vacuolar invertase genes, named MeVINV1-3, and with 653, 660 and 639 amino acids, respectively, were cloned from cassava. The motifs of NDPNG (β-fructosidase motif), RDP and WECVD, which are conserved and essential fo...

Soluble sugar composition of developing soybean seed (Glycine max L. Merr.) vary with cultivar. This study was conducted to determine the relation between soluble sugar accumulation and associated enzyme activities in developing seed of two soybean cultivars, Williams 82 and Wolverine. Activities of sucrose synthase, invertase, galactinol synthase, and a-galactosidase were compared to soluble s...

An easy to perform, rapid and precise assay for invertase is introduced. In it enzyme activity is determined spectrophotometrically from a coupled enzymatic reaction.

Plant reproduction is sensitive to water deficits, especially during the early phases when development may cease irreversibly even though the parent remains alive. Grain numbers decrease because of several developmental changes, especially ovary abortion in maize (Zea mays L.) or pollen sterility in small grains. In maize, the water deficits inhibit photosynthesis, and the decrease in photosynt...

Heterogeneity is a ubiquitous feature of biological systems. A complete understanding of such systems requires a method for uniquely identifying and tracking individual components and their interactions with each other. We have developed a novel method of uniquely tagging individual cells in vivo with a genetic 'barcode' that can be recovered by DNA sequencing. Our method is a two-component sys...

In dormant conidia of Aspergillus oryzae, alpha-amylase, invertase, and glucose dehydrogenase were induced by their respective inducers. Neither germination nor swelling occurred during this period.