Salad vegetables are the important sources of vitamins and minerals for human diet. However, salad potential vehicles non-typhoidal Salmonella spp. (NTS) as reported in our previous study. Such contamination posed significant food poisoning risk to consumer. The aims present study include determination virulence genes prevalence NTS isolates samples from retail markets farms. Besides, character...

Salmonella enterica isolates (n = 122), including 32 serotypes from 113 dogs and 9 cats, were obtained from household dogs (n = 250) and cats (n = 50) during 2012-2015. The isolates were characterized by serotyping, antimicrobial resistance phenotyping and genotyping, and virulence gene screening. Serovars Weltevreden (15.6%) and Typhimurium (13.9%) were the most common. The majority (43%) of t...

The rapid detection of pathogens in food is becoming increasingly critical for ensuring the safety of consumers, since the majority of food-borne illnesses and deaths are caused by pathogenic bacteria. Hence, rapid, sensitive, inexpensive and convenient approaches to detect food-borne pathogenic bacteria is essential in controlling food safety. In this study, a multiplex PCR assay for the rapid...

INTRODUCTION The prevalence of virulence genes in non-typhoidal Salmonella (NTS) and its association with commonly used antibiotics in West Africa is unknown. METHODOLOGY We tested 185 NTS isolates from children, animals, and food products for the presence of twelve virulence genes by PCR. Ten of the virulence genes tested belonged to the five Salmonella pathogenicity islands implicated in it...

Salmonella enterica isolates representing commonly isolated serotypes in Tunisia were analyzed using genotyping and phenotyping methods. ERIC and ITS-PCR applied to 48 Salmonella spp. isolates revealed the presence of 12 and 10 different profiles, respectively. The distribution of profiles among serotypes demonstrated the presence of strains showing an identical fingerprinting pattern. All Salm...

The prevalence and concentrations of Campylobacter jejuni, Salmonella spp. and enterohaemorrhagic Escherichia coli (EHEC) were investigated in surface waters in Brisbane, Australia using quantitative PCR (qPCR) based methodologies. Water samples were collected from Brisbane City Botanic Gardens (CBG) Pond, and two urban tidal creeks (i.e., Oxley Creek and Blunder Creek). Of the 32 water samples...

Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific genes namely ipaH1, ipaH, wbgZ, wzy an...

The introduced tree, Sapium sebiferum, is a serious invader throughout the southeastern United States. Low herbivore loads, commonly attributed to high herbivore resistance, are assumed to give Sapium a competitive advantage over native vegetation. Because herbivory resistance is considered energetically expensive, we predicted that if Sapium experienced higher levels of damage its growth would...

Infertility in recent years is a growing public health issue throughout the developed world. Assisted reproductive techniques, especially in vitro fertilization, have the poten‐ tial to partially overcome the low natural reproductive ratio. Nowadays, single embryo transfer gains grounds in clinical practice, urging the development of more reliable methods for selecting the best embryo. In the t...

Article history: Received on: 17/08/2016 Revised on: 08/09/2016 Accepted on: 07/10/2016 Available online: 29/11/2016 The present study has explored the culture-independent quantification of Salmonellae in selected food items by targeting invA gene using molecular beacon (MB) based real-time PCR assay. The assay could detect ten Genome Equivalents/PCR (GE/PCR) of reference strain S. typhimurium ...