Functional and ecological signiŽcance of rDNA intergenic spacer variation in a clonal organism under divergent selection for production rate Elena Gorokhova, Thomas E. Dowling, Lawrence J. Weider, Teresa J. Crease and James J. Elser Department of Biology, Arizona State University, Tempe, AZ 85287-1501, USA The University of Oklahoma Biological Station, HC-71, Box 205, Kingston, OK 73439, USA De...

The evolutionary history and times of divergence of triatomine bug lineages are estimated from molecular clocks inferred from nucleotide sequences of the small subunit SSU (18S) and the second internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA of these reduviids. The 18S rDNA molecular clock rate in Triatominae, and Prosorrhynchan Hemiptera in general, appears to be of 1.8% per 10...

This study examined the polymerase chain reaction (PCR) amplified internal transcribed spacer (ITS) and 26S ribosomal DNA (rDNA) regions of 15 entomopathogenic nematode isolates including Steinernema feltiae syn. bibionis, S. glaseri, seven strains of S. carpocapsae, four strains of Heterorhabditis bacteriophora, and two field isolates. RDNA length variation was not observed among the isolates ...

A new species of Botryozyma, Botryozyma mucatilis, was isolated from the surface of free-living nematodes, Panagrellus dubius, inhabiting slime flux from hybrid poplars, Populus deltoidesxtrichocarpa, in Oregon, USA. This species was discovered in relatively close proximity to the teleomorphic species Ascobotryozyma americana and Ascobotryozyma cognata, both collected from P. dubius nematodes i...

From a rRNA repeating unit (9.1 kbp) of mustard we have The DNA was sequenced according to the method of Maxam sequenced the first internal transcribed spacer, the 5.8S rRNA and Gilbert gene, and the second internal transcribed spacer [1]. Here, we present the nucleotide sequence of a 5500 bp fragment containing the 3 ' end terminus of the 25S gene (486 bp), the intergenic spacer REFERENCES (32...

The unprecedented ongoing biodiversity decline necessitates scalable means of monitoring in order to fully understand the underlying causes. DNA metabarcoding has potential provide a powerful tool for accurate and rapid monitoring. Unfortunately, many cases, lack universal standards undermines widespread application metabarcoding. One most important considerations plants, aside from selecting p...

The aim of the present study was to analyse sequences of 16S-23S internal transcribed spacer regions of different Geobacillus species, identify structural elements of these regions and evaluate the possibility to use these structural elements in the taxonomy of the genus Geobacillus. GEOBAC-PCR was used in order to obtain 16S-23S rRNA internal transcribed spacer sequences. Certain structural el...