This article explores the ways in which intensification of parenting and notion children at risk have influenced grandmothers’ narratives practices. Interviews with grandmothers who regularly look after their grandchildren, reveal that practices are framed around notions to be protected, educated entertained. Such aspects roles as protectors, educators, playmates confidants involved negotiation...

The increasing adoption of genomics and sequencing-based approaches to detect foodborne pathogens has led a new direction in food safety diagnostics. Sequencing technologies such as next-generation sequencing are used confirm the taxonomic hierarchy revision, describe novel species, genome construction, prediction microbial functions, risk assessment, identify transmission dynamics, conduct rou...

BACKGROUND Carbapenem-resistant Acinetobacter baumannii (CRAB) is associated with nosocomial infections worldwide. Here, we used phage as a potential agent to evaluate the efficacy of daily cleaning practices combined with a bacteriophage-containing aerosol against CRAB. METHODS A two-phase prospective intervention study was performed at a 945-bed public teaching hospital. From March to Decem...

BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of infection in the intensive care unit (ICU). Although surveillance culture for MRSA is recommended for ICU patients, no comparative study investigating the optimal sites and frequency of culture has been performed in this population. METHODS A prospective observational cohort study was performed i...

Cultured primary hippocampal neurons are ideal tool for investigating the subcellular localization and trafficking of neuronal proteins. The aim of the present study was to establish a method to co-culture hippocampal neurons and cortical astrocytes, which would guarantee well conditions of neurons. Newborn Sprague-Dawley (SD) rats were sacrificed by decapitation. The cortex of cerebrum was cut...

 In order to investigate the effects of artificial substrates (vertical surface of polypropylene fabrics) on cultured shrimp, we reared 28-day old Litopenaeus vannamei post-larvae (PL28) intensively for 90 days at a density of 510 shrimp/m2 in each of 8 tanks. Two tanks containing no artificial substrate were a control group, and 1, 3 and 5 artificial substrates were present in other 6 tanks. T...