Plasmids carrying 24- or 32-base-pair inserts of alternating (dG-dC) residues were used to analyze the level of methylation of the G-C-G-C sites by Hha I DNA methyltransferase and their cleavage by Hha I endonuclease in the B-DNA or Z-DNA conformation. In supercoiled plasmids in which the inserts formed Z-DNA, the extent of methylation at the insert G-C-G-C sites was dramatically lower than the...

PURPOSE The purpose of this study was to evaluate Interaural Attenuation (IA) in frequency base in the insert earphones that are used in audiological assessments. METHODS Thirty healthy subjects between 18-65 years of age (14 female and 16 male) participated in our study. Otoscopic examination was performed on all participants. Audiological evaluations were performed using the Interacoustics ...

Several pBluescript-derived plasmids of various sizes were constructed to study the effects of multicopy plasmid size on bacterial fitness and plasmid loss. Transformed and untransformed bacterial clones were grown in media with or without ampicillin. Bacterial fitness (measured by growth rate), plasmid presence or absence, and plasmid copy number were assessed during successive subculturings. ...

A high frequency of proviral acquisition has previously been reported in the offspring of SWR/J-RF/J hybrid mice. In the present study, it was investigated whether this proviral acquisition would be useful for large-scale insertional mutagenesis studies. A population of SWR/J-RF/J hybrid mice with a predominantly SWR/J background was created. Lines of mice with such a background and partially c...

Manipulating a database system on a quantum computer is an essential aim to benefit from the promising speed-up of quantum computers over classical computers in areas that take a vast amount of storage and processing time such as in databases. In this paper, the basic operations for manipulating the data in a quantum database will be defined, e.g. INSERT, UPDATE, DELETE, SELECT, backing up and ...

This paper describes Continuous Queries (CQ) in Oracle RDBMS, a feature that incorporates stream and complex event processing into an RDBMS, the first such attempt in commercial databases. The feature is based on the concept of query difference and allows us to monitor real time changes to the query as the result of changes to its underlying tables. The result of a continuous query can be depos...

Reduction is the single most common technique used in designing algorithms. Reducing one problem X to another problem (or set of problems) Y means to write an algorithm for X , using an algorithm or Y as a subroutine or black box. For example, the congressional apportionment algorithm described in Lecture 0 reduces the problem of apportioning Congress to the problem of maintaining a priority qu...

To assess the potential to generate quantitative genetic variation by insertional mutagenesis in a vertebrate, lines of mice in which many provirus vector inserts segregated at a low initial frequency on an inbred background (insert lines) were subjected to divergent artificial selection on body weight at 6 weeks and responses and heritability estimates compared to control lines lacking inserts...

We have developed an efficient strategy for cloning of PCR products that contain an unknown region flanked by a known sequence. As with ligation-independent cloning, the strategy is based on homology between sequences present in both the vector and the insert. However, in contrast to ligation-independent cloning, the cloning vector has homology with only one of the two primers used for amplific...

The hemolytic, uropathogenic Escherichia coli 536 (O6:K15:H31) contains two inserts in its chromosome (insert I and insert II), both of which carried hly genes, were rather unstable, and were deleted spontaneously with a frequency of 10(-3) to 10(-4). These inserts were not found in the chromosome of two nonhemolytic E. coli strains, whereas the chromosomal sequences adjacent to these inserts a...