The present work was finalised to develop chemiluminescent assays more sensitive and easy to use than the analytical methods currently in use to detect traces of TNT (2,4,6-trinitrotoluene), TATP (Triacetone triperoxide) and HMTD (Hexamethylene triperoxide diamine). For TNT detection we developed an indirect competitive ELISA with chemiluminescent end-point (CL-ELISA) and a Lateral Flow ImmunoA...

Serum antibodies against human T cell leukaemia virus type I (HTLV-I) were investigated in 12 patients by four methods: indirect immunoperoxidase staining, indirect immunofluorescence, enzyme linked immunosorbent assay (ELISA), and strip radioimmunoassay based on the Western blotting assay. Seven patients had systemic lupus erythematosus (SLE) and five various autoimmune diseases with one or mo...

MAROBELA·RABOROKGWE. C., NICHOlAS, R. , AYLING , R. & BASHIRUOOIN, J .S. 2003. Comparison of complement fixation test, Irrmunoblotting, indirect ELISA, and compelitive ELISA for detecting antibodies to Mycoplasma mycoldes subspecies mycokJes small colony (SC) in naturally Infected cattle from the 1995 outbreak in Botswana. Onderstepoort Journal of veterinary Research, 70:21 27 Four serological ...

AIM This study was taken up to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for screening antibodies against Leptospira spp. in canines. MATERIALS AND METHODS An i-ELISA was developed using outer membrane protein extracted from Leptospira interrogans serovar canicola used for coating the well with concentration of 0.5 µg/µl. A total of 250 serum samples from clinically affe...

A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC(50)) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related mole...