background: the excellent biocompatibility, biodegradability and biological properties of the hydrogels, fabricated using natural polymers, especially polysaccharides, are very advantageous for biomedical applications. gum tragacanth (gt) is a heterogeneous highly branched anionic polysaccharide, which has been used extensively in food and pharmaceutical industries. despite,  its desirable prop...

In situ measurements of the hydrothermal formation of LiMn2O4 (LMO) nanocrystallites reveal that the reaction progresses in steps, each creating a different crystalline phase. The reaction route is summarized as KMnO4→disordered δ-MnO2→(ordered δ-MnO2)→LiMn2O4→(γ-Mn2O3)→Mn3O4. The phase purity of LMO can be controlled by reaction time and temperature where phase pure LMO is obtained after 150-2...

CHOI ET AL. l DESCRIBE PROGRAMMABLE IN SITU AMPLIFIERS based on the mechanism of hybridization chain reaction (HCR) that enable simultaneous mapping of multiple target mRNAs within whole-mount zebrafish embryos. In contrast to traditional in situ hybridization approaches, HCR in situ amplification is performed for all target mRNAs simultaneously, so the experimental timeline is independent of t...

The ability to design artificial extracellular matrices as cell-instructive scaffolds has opened the door to technologies capable of studying the fate of cells in vitro and to guiding tissue repair in vivo. One main component of the design of artificial extracellular matrices is the incorporation of biochemical cues to guide cell phenotype and multicellular organization. The extracellular matri...

PURPOSE Little information exists about the possible influence of serum HER2/neu on response to chemotherapy. We propose that the assessment of serum HER2/neu in a pretreatment serum sample may be useful in predicting response to neoadjuvant chemotherapy. METHODS All breast cancer patients were tested by immunohistochemical stain and fluorescent in situ hybridization for HER2/neu before treat...

The formation of the layered double hydroxide [Cu2Cr(OH)6]Cl·yH2O from the reaction between CuO and aqueous CrCl3·6H2O was explored using synchrotron X-ray diffraction and ex situ analyses. The use of hard X-rays permitted time-resolved in situ studies to be performed as the reaction proceeded under a range of conditions. Additional information was obtained from ex situ experiments in which ali...

We demonstrate the application of Compressed Sensing-NMR to decrease the data acquisition time of 2D COSY NMR from >5 h to ∼1.5 h such that the kinetics of a reaction are followed, along with identification of intermediate and product species.