AIM To localise the recently discovered glycoprotein opticin in the adult human eye. METHODS Polyclonal rabbit antisera were raised against two different opticin peptides. Isolated human vitreous collagen fibrils were extracted with 8 M urea and the extract analysed by SDS-PAGE and western blotting. Paraffin embedded sections from two normal eyes were subjected to immunohistochemical analysis...

BACKGROUND/AIMS Carbomers are widely used in products for the treatment of dry eye; however, the polymer gel thins on addition of probes (for example, fluorescein salt) confounding the comparison of products by objective clinical tests such as spectrophotofluorimetry or scintigraphy. A novel method of radiolabelling carbomer gels, with minimum change to their rheology, has permitted the non-inv...

the nutritive value of pistachio epicarp (pe) was evaluated by in situ and in vitro techniques. chemical analysis indicated that pe was high in crude protein (11.30%) and low in neutral detergent fiber (26.20%). total phenols, total tannins, condensed tannins and hydrolysable tannins contents in pe were 8.29%, 4.48%, 0.49% and 3.79%, respectively. ruminal dry matter and crude protein degradatio...

PURPOSE To determine the distribution of platelet-activating factor (PAF) receptor in the rat eye. METHODS Platelet-activating factor receptor messenger RNA (mRNA) expression was evaluated with reverse transcription-polymerase chain reaction (RT-PCR) in RNAs from several rat ocular tissues. Distribution of the expression was determined with in situ hybridization in adult rat eye sections and ...

copper matrix composite reinforced by 1wt.% tib2 particles was prepared using in situ reaction of cu-1.4wt.% ti and cu-0.7wt.% b by rapid solidification and subsequent heat treatment for 1-20 hrs at 900ºc. high-resolution transmission electron microscopy (hrtem) characterization showed that primary tib2 particles were formed in liquid copper. heat treatment of as-solidified samples led to the f...

the first purpose of this study is to present an alternative robust model in order to describe ruminal degradation kinetics of forages and to minimize the fitting problems. for this purpose, the korkmaz-uckardes (ku) model, which has a logarithmic structure, was developed. the second purpose of this study is to estimate, by using the korkmaz-uckardes (ku)model, the parameters tp (the time to pr...

degradation of dry matter (dm) and nitrogen (n) in fresh or frozen-thawed alfalfa and ryegrass was studied by using in situ technique. the forages were labeled with 15n during growth in a glasshouse, harvested at similar growth phase, and fresh (f) or frozen-thawed (ft) samples were incubated in the rumen of 3 sheep. there was no difference (p>0.05) between forage type (s) for the immediately s...

in this work, the potential of auto-ignition of heavy oil during in-situ combustion (isc) process was studied. kinetic studies were carried out using thermo gravimetric analyzer (tga), differential scanning calorimetry (dsc) and accelerating rate calorimetric (arc) techniques. effects of oxygen partial pressure, reservoir pressure and clay on auto ignition condition were investigated on a numbe...