Microdissection techniques allow a cell-type or even cell-specific mRNA analysis within complex tissues. Furthermore, valid mRNA quantitation can be performed by real-time reverse transcriptase-polymerase chain reaction from a few isolated cells obtained from cryosections. For a more precise access to many cell types, this technique has to be complemented by a cell-type-specific immunostaining....

1 Immune Disease Institute and Program in Cellular and Molecular Medicine at Children's Hospital, D-239, 200 Longwood Avenue, 02115, Boston, MA, USA 2 Department of Pathology, Harvard Medical School, D-239, 200 Longwood Avenue, 02115, Boston, MA, USA 3 Hematology Scientific Center, 4 Novyi Zykovski Proezd, 125167, Moscow, Russia 4 A.N.Belozersky Institute of Physico-Chemical Biology, Moscow Sta...

High-resolution 3D reconstruction and morphometric analysis of striosomes was carried out in macaque monkeys by using immunocytochemistry for the Kv4 potassium channel subunit potassium channel interacting protein 1 (KChIP1), a novel marker. The striosomes form a connected reticulum made up of two distinct planar sheets spanning several millimeters in the putamen, and long finger-like branches ...

With the advent of new monoclonal antibodies that are applicable to formalin-fixed, paraffin embedded sections, immunophenotyping is becoming increasingly important in the diagnosis and classification of lymphomas. However, multiple factors such as fixation, trypsinization and even type of antibodies used have certain effects on the final outcome of the staining procedure. In this paper we repo...

Primary neurogenesis is a dynamic and complex process during embryonic development that sets up the initial layout of the central nervous system. During this process, a portion of neural stem cells undergo differentiation and give rise to the first populations of differentiated primary neurons within the nascent central nervous system. Several vertebrate model organisms have been used to explor...

Aims—The nuclear enzyme DNA topoisomerase II has been shown to be required for chromatin condensation and chromosomal segregation during mitosis; its isoform topo IIá is linked with active cell proliferation in mammalian cells. The aim of this study was to examine the relation of the expression of topo IIá to the biological behaviour of conventional urinary bladder cancer. Methods—Formalin fixe...

Immunocytochemistry on sectioned tissue is a powerful method for determining the cellular and subcellular distributions of an epitope. However, where the expression is patterned, understanding the distribution often requires extensive, time-consuming threedimensional reconstruction with all its attendant artifacts. The techniques described in this unit circumvent these problems. The distributio...