OBJECTIVE Autoantibodies directed against the aminoacyl tRNA synthetases are associated with myositis, arthritis, Raynaud's phenomenon, mechanic's hands, fever and interstitial pneumonia, clinically referred to as the anti-synthetase syndrome (ASS). The aim of this study was to characterize the autoantibody profile in a patient with clinical features of ASS whose routine diagnostic testing was ...

Concentration of iron in plasma, total iron-binding capacity (TIBC), and transferrin saturation are often determined by standard spectrophotometric methods, but iron concentration may be quantified by immunoprecipitation or, electrochemically, by controlled-potential coulometry. Because these iron assays do not all measure the same form(s) of iron, we studied subjects in various states of iron ...

Immunoprecipitation (IP) and Co-IP are common techniques for isolating proteins and identifying protein-protein interactions, respectively. Exclusion-based sample preparation (ESPTM) with EXTRACTMAN® (Figure 1) allows for rapid, yet gentle washing of protein solutions during these procedures. EXTRACTMAN has been shown to be ideal for isolating elusive, weakly-bound protein complexes by Co-IP us...

We have taken a new approach to the identification of E2F-regulated promoters. After modification of a chromatin immunoprecipitation assay, we cloned nine chromatin fragments which represent both strong and weak in vivo E2F binding sites. Further characterization of three of the cloned fragments revealed that they are bound in vivo not only by E2Fs but also by members of the retinoblastoma tumo...

Abstract Background As a novel type of non-coding RNA, circular RNAs (circRNAs) play critical role in the initiation and development various diseases, including cancer. However, exact function circRNAs human cervical cancer remains largely unknown. Methods We identified circRNA signature upregulated between paired adjacent normal tissues. Using two different cohorts GEO database, total six were...

Abstract Background Sheath blight disease (ShB) is a destructive affecting rice production. Previously, we have reported that Loose Plant Architecture 1 (LPA1) promotes resistance to ShB. However, the mechanisms by which LPA1 confers against this not been extensively investigated. Notably, interactors regulate LPA-1 activity remain elusive. Findings In study, identified interaction of kinesin-l...