background tissue engineering is used for the treatment of many diseases, and the ideal cell source for cartilage tissue engineering is chondrocytes. the main limitation of chondrocyte is the low number of cells in cartilage tissue engineering. this study investigated a suitable cell source with high proliferation rate to obtain a large number of chondrocytes. methods adult cartilage tissue sam...

Microwave irradiation during tissue fixation and immunostaining reduces the sample preparation time. Microwave irradiation also facilitates the penetration of fixatives and antibody solutions into the tissues, resulting in efficient fixation and reduction of non-specific antibody binding, respectively. Experimental procedures involving immunofluorescence microscopy are time-consuming as this me...

Mast cells (MCs) is an effector in inflammatory skin diseases by secreting potent mediators. It has been proved that MCs significantly increase and activate the lesions of psoriasis patients, which can be activated external factors a variety endogenous mediators to participate pathological changes formation microenvironment psoriasis. Current studies suggest axis activation releasing factors, s...

In previous work we used immunofluorescent staining with specific antibodies to study the distribution of five desmosomal antigens in the epithelia of different vertebrate animals. We showed that all five antigens were present in all epithelia studied in human, bovine, rat, guinea pig, chick and frog (Rana pipiens) tissues. It was concluded that desmosomes are highly conserved structures. This ...

BACKGROUND Tissue engineering is used for the treatment of many diseases, and the ideal cell source for cartilage tissue engineering is chondrocytes. The main limitation of chondrocyte is the low number of cells in cartilage tissue engineering. This study investigated a suitable cell source with high proliferation rate to obtain a large number of chondrocytes. METHODS Adult cartilage t...

An immunofluorescent technique was used to demonstrate soluble adenoviral antigens in epithelial cells on conjunctival scrapings of patients with epidemic keratoconjunctivitis (EKC). Conjunctival scrapings for immunofluorescence and viral isolation studies were performed on 79 patients suspected of having EKC or other acute follicular or papillary conjunctivitides. Of 41 patients with clinical ...