Some enzymes were immobilized in an inner layer of a filter paper using the osmotic action of a contact dehydrating sheet (CDS). CDS was examined to determine if the efficiency and rapidity of an enzyme reaction could be improved, thereby making better of the preparation of an immobilized-enzyme membrane. The activity of the membrane, in which an enzyme exists in its layer, was compared with th...

BACKGROUND Biocatalytic processes often require a full recycling of biocatalysts to optimize economic benefits and minimize waste disposal. Immobilization of biocatalysts onto particulate carriers has been widely explored as an option to meet these requirements. However, surface properties often affect the amount of biocatalysts immobilized, their bioactivity and stability, hampering their wide...

A method for immobilization of papain has been selected based on the interaction between its histidine, cysteine and tryptophan residues with the immobilized metal ion (IMI) carrier for maximum binding on a small volume of the carrier. The immobilized papain retained high activity has improved thermal stability and the carrier could be recovered from the spent bound enzyme, to be reused. Reimmo...

The biochemical properties of invertase entrapped in alginate gel were studied. The kinetic parameters were determined for immobilized and free invertase. The value of Michaelis constant Km of the immobilized invertase (139.19 mM) was greater than that of the free invertase (93.19 mM), whereas, Vmax was smaller for the immobilized enzyme. Immobilization impressively improved the thermal and sto...

A continuous-flow clinical analyzer for the routine estimation of urea is described that makes use of an immobilized-enzyme nylon-tube reactor as part of a flow-through system (a Technicon AutoAnalyzer I). Results of blood-urea analyses by use of the immobilized urease are compared with determinations made with the diacetyl monoxime method and the urease solution method. Clinical trials carried...

This paper reports the covalent immobilization of penicillin G acylase from E. coli on sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes kinetic properties of the immobilized enzyme. The selected enzyme belongs to a class of biocatalysts whose industrial interest is due to their versatility to mediate hydrolysis of penicillins and semi-synthetic β−lactam antibiotics syn...

The occurrence of emerging contaminants, such as estrogens, in secondary and tertiary treated effluents sewage-impacted water bodies is one the major obstacles to implementation reuse. This review critically evaluates performance process enzymatic degradation its efficiency. data collected from peer-review literature show that enzymes have been extensively applied (in both free immobilized form...

E. Horozova*, N. Dimcheva and Z. Jordanova Department of Physical and Theoretical Chemistry, University of Plovdiv 24, Tsar Assen St., Plovdiv 4000, Bulgaria Z. Naturforsch. 52c, 639-644 (1997); received May 5/July 3, 1997 Catalase, Immobilized Enzyme, Carbon Materials, Electrochemical and Biocatalytic Activity The adsorption of catalase on two types of soot differing in their structure has bee...

β-Galactosidase (β-gal) was immobilized by covalent binding on novel κ-carrageenan gel beads activated by two-step method; the gel beads were soaked in polyethyleneimine followed by glutaraldehyde. 2(2) full-factorial central composite experiment designs were employed to optimize the conditions for the maximum enzyme loading efficiency. 11.443 U of enzyme/g gel beads was achieved by soaking 40 ...

Numerous types of nanoparticles and nanocomposites have successfully been employed for the immobilization and stabilization of amylolytic enzymes; α-amylases, β-amylases, glucoamylases and pullulanases. Nano-support immobilized amylolytic enzymes retained very high activity and yield of immobilization. The immobilization of these enzymes, particularly α-amylases and pullulanases, to the nanosup...