INTRODUCTION Stereoselective chemical reactions have become extremely important in both academic and industrial settings for the synthesis of useful molecules. Catalytic reactions are of particular importance due to their capacity to effect otherwise impossible transformations using substoichiometric quantities of the catalyst. Traditionally, transition metal catalysts have played a central rol...

The challenging field of de novo enzyme design is beginning to produce exciting results. The application of powerful computational methods to functional protein design has recently succeeded at engineering target activities. In addition, efforts in directed evolution continue to expand the transformations that can be accomplished by existing enzymes. The engineering of completely novel catalyti...

This study implements a convenient microreactor for biocatalysis with enzymes immobilized on magnetic nanoparticles (MNPs). The enzyme onto MNPs by adsorption or covalent bonds was lipase B from Candida antarctica (CaLB). were obtained covering the magnetite core silica shell and later hexadecyltrimethoxysilane, while immobilization, silica-covered functionalized layer forming mixtures of hexad...

Alkaline Protease enzyme produced from Bacillus Licheniformis PTCC1331 in optimum Condition(Temperature 37 ?C, 150 rpm, PH=10), is immobilized in different concentrations of Sodium Alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. Maximum enzymatic activity of 46 was obtained in 2%w/v Sodium alginate solution. Investiga...

Abstract Purpose This work studies the immobilization of two enzymes, alcohol dehydrogenase (ADH) and aldehyde (AldDH) both from Saccharomyces cerevisiae , which could be used to produce high value-added molecules carboxylic acids embedded in anaerobic digestate. Methods In particular, three mesoporous siliceous materials, with different specific surface areas pore sizes, (MSU-H, MSU-F MCF 0.75...

The enzymatic kinetic resolution (EKR) of racemic alcohols or esters is a broadly recognized methodology for the preparation these compounds in optically active form. Although EKR approaches have been developed enantioselective transesterification vast number secondary hydrolysis their respective esters, to date, there no report bio- chemo-catalytic asymmetric synthesis non-racemic possessing 1...

This study mainly aims to find the optimal conditions for immobilizing a non-commercial β-glucosidase from Aspergillus niger via cross-linked enzyme aggregates (CLEAs) by investigating effect of cross-linking agent (glutaraldehyde) concentration and soy protein isolate/enzyme ratio (or spacer/enzyme ratio) on catalytic performance through central composite rotatable design (CCRD). The influence...

an enzyme electrode was developed using the enzyme sulfite oxidase (ec1.8.3.1) immobilized onto pig intestine. hydrogen peroxide produced was monitored amperometrically. all experimental parameters such as ph, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. the response to sulfite concentration was linear in the range 5.2´10-6 to 1.02´...