BACKGROUND GJA1 and PTX3 were proposed as gene markers for oocyte and embryo developmental competence, while SERPINE2 was reported to be associated with pregnancy outcome. PRSS35, which is exclusively expressed in the ovary, may be correlated with oocyte competence. This study was conducted to evaluate the correlation of cumulus GJA1, PRSS35, PTX3, and SERPINE2 gene expression levels with oocyt...

This case report describes the birth of a healthy infant after cryopreservation of zygotes produced by in-vitro matured oocytes retrieved from an anovulatory woman with polycystic ovarian syndrome (PCOS). To initiate the treatment cycle, the patient received intravaginal progesterone at night for 10 days to induce a withdrawal bleed. Oocyte retrieval was performed on day 11 following a withdraw...

Genital tract abnormalities and adverse pregnancy outcome are well known in women exposed in utero to diethylstilboestrol (DES). Data about adverse reproductive performance in women exposed to DES have been published, including controversial reports of menstrual dysfunction, poor responses after ovarian stimulation, oocyte maturation and fertilization abnormalities. We compared oocyte quality, ...

Detailed expression analysis of the Norway spruce (Picea abies [L.] Karst) Viviparous 1 (Pavp1) and p34cdc2 (cdc2Pa) genes was carried out during somatic embryogenesis. Pavp1, a gene associated with embryo development, was expressed in proliferating embryogenic suspension cultures in the absence of exogenous ABA. When somatic embryo formation was promoting by blocking proliferation, Pavp1 expre...

background: most studies on anabolic-androgenic steroids abuse have been done in adult rats, but few data are available to immature. objective: this study was conducted to assay the effect of nandrolone decanoate (nd) on the testis and testosterone concentration in male immature rats compare with mature ones in short and long time. materials and methods: 40 mature rats were divided into 4 group...

the laelapid mite, gaeolaelapsaculeifer (canestrini) is widespread in soil habitats and feeds on different small arthropods, fungi and nematodes. the development and fecundity of g. aculeifer feeding on rhizoglyphus echinopus (fumouze & robin) as prey was studied at eight different constant temperatures which include: 16, 17.5, 20, 22.5, 25, 27.5, 30 and 32.5 ºc, with relative humidity of 6...

The birth rate of embryos produced in vitro (IVF) is still lower than that vivo. Three major steps for the success IVF technique are maturation immature oocytes, fertilization matured and culture resulting embryos. Studying mRNA expression early embryonic development stages important can help to assess embryo quality optimize production protocols vitro. current study aimed determine levels deve...

Embryogenesis in plants can commence from cells other than the fertilized egg cell. Embryogenesis initiated from somatic cells in vitro is an attractive system for studying early embryonic stages when they are accessible to experimental manipulation. Somatic embryogenesis in Arabidopsis offers the additional advantage that many zygotic embryo mutants can be studied under in vitro conditions. Tw...

Background: Polycystic ovary syndrome (PCOS) is a frustrating experience for women, considered as a complex challenge for researchers and clinicians. Despite the enormous effort to define the cause of PCOS, the etiology and pathogenesis still remain unclear. Oocyte quality is reflected in the oocyte’s intrinsic developmental potential. Studies have shown that the follicular development is disru...

BACKGROUND The effect of hCG priming on oocyte maturation and subsequently outcome in IVM cycles has remained a debated issue. A randomized controlled study was performed to investigate whether or not hCG priming prior to oocyte aspiration can improve the developmental competence of immature oocytes from unstimulated ovaries in women with polycystic ovarian syndrome (PCOS). METHODS Eighty two...