To assess the involvement of miRs in the inflammatory reflex, we used FVB/N mice as well as murine RAW 264.7 macrophage-derived cells which are known to respond to LPS by production of proinflammatory mediators such as Nitric Oxide (NO) and cytokines such as IL1β, IL6, and IL-12; primary human macrophages obtained from healthy donors, which can mimic more closely the reaction of nontumor cells ...

We report the effect of recombinant human interleukin-1β (IL-1β) on cell morphology and on the pattern of matrix metalloproteinase (MMP) gelatinolytic activity expressed by quiescent and growing human osteoarthritic chondrocytes. After treatment with IL1β, gelatin zymography revealed a significant increase of 64-62 kDa MMP-2 active forms suggesting an overexpression of its activator membrane ty...

Objectives: Femoroacetabular impingement (FAI) is considered a leading cause of early hip OA, but initial molecular mechanisms that lead FAI patients to OA progression remains be elucidated. Recent articles revealed epigenetic changes are associated with progression, specifically, DNA methyltransferase 3B (DNMT3B) plays critical role on cartilage homeostasis by attenuating catabolic gene expres...

(1) Background: Intrinsic characteristics of the implant surface and possible presence endotoxins may affect bone–implant interface cause an inflammatory response. This study aims to evaluate response induced in vitro macrophages contact with five different commercially available dental implants. (2) Methods: one zirconia NobelPearl® (Nobel Biocare) four titanium implants, Syra® (Sweden & M...

Purpose: This study investigates the anti-melanogenic effects of lotus seed and seedpod extract in B16F10 murine melanoma cells, demonstrates possible mechanisms involved anti-pigmentation.Methods: The extracts were prepared using 70% ethanol as solvent. irritation potential was investigated water-soluble tetrazolium salt (WST-1)-based cytotoxicity assay expression analysis pro-inflammatory cyt...