INTRODUCTION Currently available clinical assays to detect antiphospholipid antibodies (aPL) test for IgG and IgM antibodies to cardiolipin (aCL) and β2-glycoprotein I (aβ2GPI). It has been suggested that testing for IgA aPL and for antibodies to Domain I (DI), which carries the key antigenic epitopes of β2GPI, could add value to these current tests. We performed an observational, multicenter c...

Rabbit immunoglobulin (Ig)M and IgG antibodies to Brucella abortus and Bordetella pertussis were isolated as purified products and their specific secondary biological activities were compared. IgM antibodies were found to be more active than IgG proteins in inducing agglutination and sensitization of B. abortus for the complement-dependent bactericidal effect and in inhibiting B. pertussis-indu...

A Mouse polyclonal antiserum against Trypanosoma cruzi or its IgG and IgM fractions and five monoclonal antibodies (two IgM, two IgG(1) and one IgG(2a)) recognize and combine with membrane components of trypomastigote forms of the parasite as revealed by immunofluorescence. Although all these antibodies sensitize trypomastigotes and prepare them to activate the complement (C) system, as measure...

based on serological,toxoplasmosis infection is a widespread disease.with regard to this fact and because of the occurence of congenital toxoplasmosis infections due to asymptomatic infections in pregnant women,determination of the rate of toxoplasma antibodise(IgG,IgM) before pregnancy and finding the involved factors in increasing toxoplasmosis prevalence seems to be necessary.in this cross s...

Serum immunoglobulin (Ig)M provides the initial response to foreign antigen and plays a regulatory role in subsequent immune response development, accelerating the production of high-affinity IgG. Here we show that mice deficient in serum IgM have an increased propensity to spontaneous autoimmunity as judged by the development with age of serum IgG anti-DNA antibodies and the renal deposition o...

Primary, secondary and even tertiary dengue infections can be detected taking into account the existence of four serotypes. During a primary infection, individuals develop IgM antibodies 5–6 days after the onset of illness lasting 30 to 90 days. IgG levels rise after IgM and are detectable for life[7]. During a secondary infection IgM levels are lower and in some cases absent. In contrast, IgG ...

Acquired protection from Plasmodium falciparum malaria takes years to develop, probably reflecting the ability of the parasites to evade immunity. A recent example of this is the binding of the Fc region of IgM to VAR2CSA-type PfEMP1. This interferes with specific IgG recognition and phagocytosis of opsonized infected erythrocytes (IEs) without compromising the placental IE adhesion mediated by...

INTRODUCTION Systemic lupus erythematosus is characterized by production of autoantibodies to RNA or DNA-protein complexes such as small nuclear ribonucleoproteins (snRNPs). A role of Epstein-Barr virus in the pathogenesis has been suggested. Similar to Epstein-Barr virus, cytomegalovirus (CMV) infects the majority of individuals at a young age and establishes latency with a potential for react...

conclusions according to the results, brucellosis has remained endemic in this region. most cases were detected by elisa igg. the highest kappa agreements were between tests krb-sat, krb-igg and ksat-igg, while the lowest levels of agreement were between tests sat-igm and elisa igm-igg. considering that elisa igm results are covered by sat and elisa igg test results, applications of this test d...

Immunoglobulins (Ig) in feline sera and secretions were identified by immuno-electrophoresis and immunodiffusion with rabbit antisera prepared to feline IgG, IgA, IgM, and whole serum. Adult cat sera, colostral whey, postcolostral sera, tears, and nasal secretions contained IgG, IgA, and IgM. IgG was the only Ig identified in precolostral sera and cerebrospinal fluid. Milk, intestinal contents,...