infectious bronchitis (ib) and newcastle disease (nd) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. in the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-rt-pcr. rna was extracted...

INTRODUCTION The first H5N1 outbreak in Burkina Faso was reported to the World Organization for Animal Health on 3 April 2006. This study aimed to determine the prevalence of avian influenza virus, infectious bronchitis virus, and Newcastle disease virus among domestic and wild birds in highly pathogenic avian influenza (HPAI) H5N1 outbreaks areas. METHODOLOGY We collected paired tracheal and...

infectious bronchitis virus (ibv) is an acute and contagious viral disease of poultry that affects different systems, including the respiratory tract in particular. ibv causes major economic losses in the poultry industry globally. due to antigenic variation of the causative agent, control of the disease is difficult. to control the disease, many vaccines that belong to different serotypes are ...

The aim of this study was to provide information on the molecular characteristic and the phylogenic relationship of infectious bronchitis viruses (IBV) strains in Bushehr province in comparison to other strains reported in the Middle East. Samples were collected from broiler flocks in Bushehr province during 2014 - 2015. These flocks had respiratory problems such as gasping, sneezing and bronch...

OBJECTIVE To study the characteristics of balance performance in a sample of patients with increasing postural instability after acquired brain injury (ABI) and to establish the clinical utility of a new computerized posturographic system (NedSVE/IBV). METHODS This study included 108 patients with ABI divided into five groups from minimal to severe postural impairment. All patients were asses...

The aim of this study was to determine an infectious bronchitis (IB) infection, caused by Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop egg production soft-shelled eggs eventually death were observed flock. Clinical samples from four diseased chickens examined for the dete...

  In order to identify predominant circulating types of infectious bronchitis(IB) virus in the period of 1999-2004 in Iran, samples(trachea, lung, kidney) from 150 various flocks showing respiratory signs suspected to be related to IB infection were inoculated to SPF eggs. Then allantoic fluids studied by RT-PCR and Nested PCR using group and type specific primers to probing Massachusset, 793/B...

BACKGROUND: Avian infectious bronchitis is considered as an important viral disease worldwide. Genotyping based on the S1 subunit of spike protein gene of the causative agent, avian infectious bronchitis virus, can be used to classify IBV isolates. Objective: This survey was carried out to characterize the infectious bronchitis virus (IBV) genotypes circulating in Iran and determine their preva...

Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...

Simple assay systems for infectivity titrations of avian infectious bronchitis virus (IBV) in chicken embryo trachea organ cultures (OC) were developed using plastic multiplate wells with one tracheal ring per well; these assays appeared to be much more satisfactory than the conventional rolled-tube method. The medium, 0.05 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered ...