Spinosad (spinosyn A and spinosyn D), the secondary metabolite produced by Saccharopolyspora spinosa, is a potent insecticide with low effects on environment mammals. Strategies such as metabolic engineering, mutagenesis fermentation process optimization have been employed for its production enhancement. Quantitative real-time polymerase chain reaction(qRT-PCR) one of preferred methods evaluati...

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

present investigation was carried out to identify dna polymorphism of il-2rγ and chb6 genes. sixty five birds belonging to the indigenous chicken of himachal pradesh were utilized. good quality dna samples were subjected to pcr-rflp analysis using chicken specific primers. overnight restriction enzyme digestion was carried out at 37°c with iu hph i and pvu ii for il-2rγ and chb6 genes, respecti...