نتایج جستجو برای: hydroxybutyrate phb

تعداد نتایج: 4992  

Journal: :Biomaterials 2012
Yung-Chih Kuo Min-Jung Huang

The potential of constructs comprising induced pluripotent stem (iPS) cells and biopolymers can be high for neurological surgery practice, if the systematic activity of neuronal regeneration is clarified. This study shows a guided differentiation of iPS cells toward neurons in neuron growth factor (NGF)-grafted poly(ε-caprolactone) (PCL)-poly(β-hydroxybutyrate) (PHB) scaffolds. The porosity of ...

2017
SAEED KARBASI ZAHRA MOHAMMAD ALIZADEH

Poly(3-hydroxybutyrate) (PHB)/chitosan electrospun scaffold was recently prepared for cartilage tissue engineering purpose. The drawback of this scaffold was its low mechanical properties. This study was carried out to see if addition of multi-wall carbon nanotubes (MWNTs) to PHB/chitosan polymeric blend can show better mechanical and structural properties. To do this, three different amounts o...

2017
L.P.S. Alves A.T. Almeida L.M. Cruz F.O. Pedrosa E.M. de Souza L.S. Chubatsu M. Müller-Santos G. Valdameri

The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Az...

Journal: :Journal of bacteriology 2000
M Castañeda J Guzmán S Moreno G Espín

Azotobacter vinelandii produces two polymers: the extracellular polysaccharide alginate and the intracellular polyester poly-beta-hydroxybutyrate (PHB). A cosmid clone (pSMU588) from an A. vinelandii gene library diminished alginate production by A. vinelandii mucoid strain ATCC 9046. The nucleotide sequence and predicted amino acid sequence of the locus responsible for the mucoidy suppression ...

Journal: :Applied and environmental microbiology 2006
Birgit Gebauer Dieter Jendrossek

Two methods for accurate poly(3-hydroxybutyrate) (PHB) depolymerase activity determination and quantitative and qualitative hydrolysis product determination are described. The first method is based on online determination of NaOH consumption rates necessary to neutralize 3-hydroxybutyric acid (3HB) and/or 3HB oligomers produced during the hydrolysis reaction and requires a pH-stat apparatus equ...

2017
Wei Tao Li Lv Guo-Qiang Chen

BACKGROUND Clustered regularly interspaced short palindromic repeats interference (CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model microorganism Halomonas species TD01 has been developed as a promising industrial producer of polyhydroxyalkanoates (PHA), a family of biodegradable polyesters accumulated by bacteria as a carbon and energy reserve compound. A c...

Journal: :Applied and environmental microbiology 1997
K Kasuya Y Inoue T Tanaka T Akehata T Iwata T Fukui Y Doi

Comamonas acidovorans YM1609 secreted a polyhydroxybutyrate (PHB) depolymerase into the culture supernatant when it was cultivated on poly(3-hydroxybutyrate) [P(3HB)] or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] as the sole carbon source. The PHB depolymerase was purified from culture supernatant of C. acidovorans by two chromatographic methods, and its molecular mass was det...

2016
Meijuan Xu Jingru Qin Zhiming Rao Hengyi You Xian Zhang Taowei Yang Xiaoyuan Wang Zhenghong Xu

BACKGROUND Corynebacterium crenatum SYPA 5 is the industrial strain for L-arginine production. Poly-β-hydroxybutyrate (PHB) is a kind of biopolymer stored as bacterial reserve materials for carbon and energy. The introduction of the PHB synthesis pathway into several strains can regulate the global metabolic pathway. In addition, both the pathways of PHB and L-arginine biosynthesis in the cells...

Journal: :Biomacromolecules 2007
Xian Jun Loh Suat Hong Goh Jun Li

New biodegradable multiblock amphiphilic and thermosensitive poly(ether ester urethane)s consisting of poly[(R)-3-hydroxybutyrate] (PHB), poly(ethylene glycol) (PEG), and poly(propylene glycol) (PPG) blocks were synthesized, and their aqueous solutions were found to undergo a reversible sol-gel transition upon temperature change at very low copolymer concentrations. The multiblock poly(ether es...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید