نتایج جستجو برای: human polyclonal antibody

تعداد نتایج: 1764337  

2013
Natalie E. Stevens Cara K. Fraser Mohammed Alsharifi Michael P. Brown Kerrilyn R. Diener John D. Hayball

Passive immunotherapies utilising polyclonal antibodies could have a valuable role in preventing and treating infectious diseases such as influenza, particularly in pandemic situations but also in immunocompromised populations such as the elderly, the chronically immunosuppressed, pregnant women, infants and those with chronic diseases. The aim of this study was to optimise current methods used...

Journal: :Chemosensors 2021

The main goal of this study was to apply magnetic bead surface functionalization in the form immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) water samples. technique efficiency compared a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were car...

Journal: :Biophysical journal 2004
Lilia A Chtcheglova George T Shubeita Sergey K Sekatskii Giovanni Dietler

A new method of direct and continuous measurement of the spring constant of single molecule or molecular complex is elaborated. To that end the standard force spectroscopy technique with functionalized tips and samples is combined with a small dithering of the tip. The change of the dithering amplitude as a function of the pulling force is measured to extract the spring constant of the complex....

Journal: :Journal of bacteriology 2002
Makoto Miyata William S Ryu Howard C Berg

The effects of temperature and force on the gliding speed of Mycoplasma mobile were examined. Gliding speed increased linearly as a function of temperature from 0.46 microm/s at 11.5 degrees C to 4.0 microm/s at 36.5 degrees C. A polystyrene bead was attached to the tail of M. mobile using a polyclonal antibody raised against whole M. mobile cells. Cells attached to beads glided at the same spe...

2017
Nicole A Doria-Rose Han R Altae-Tran Ryan S Roark Stephen D Schmidt Matthew S Sutton Mark K Louder Gwo-Yu Chuang Robert T Bailer Valerie Cortez Rui Kong Krisha McKee Sijy O'Dell Felicia Wang Salim S Abdool Karim James M Binley Mark Connors Barton F Haynes Malcolm A Martin David C Montefiori Lynn Morris Julie Overbaugh Peter D Kwong John R Mascola Ivelin S Georgiev

Computational neutralization fingerprinting, NFP, is an efficient and accurate method for predicting the epitope specificities of polyclonal antibody responses to HIV-1 infection. Here, we present next-generation NFP algorithms that substantially improve prediction accuracy for individual donors and enable serologic analysis for entire cohorts. Specifically, we developed algorithms for: (a) sel...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2010
Elisabetta Bianchi Joseph G Joyce Michael D Miller Adam C Finnefrock Xiaoping Liang Marco Finotto Paolo Ingallinella Philip McKenna Michael Citron Elizabeth Ottinger Robert W Hepler Renee Hrin Deborah Nahas Chengwei Wu David Montefiori John W Shiver Antonello Pessi Peter S Kim

Eliciting a broadly neutralizing polyclonal antibody response against HIV-1 remains a major challenge. One approach to vaccine development is prevention of HIV-1 entry into cells by blocking the fusion of viral and cell membranes. More specifically, our goal is to elicit neutralizing antibodies that target a transient viral entry intermediate (the prehairpin intermediate) formed by the HIV-1 gp...

Journal: :International immunology 1999
M Astori D Finke O Karapetian H Acha-Orbea

The neonatal immune response is impaired during the first weeks after birth. To obtain a better understanding of this immaturity, we investigated the development of T cell interactions with B cells in mice. For this purpose, we analyzed the immune response to three T-dependent antigens in vivo: (i) the polyclonal antibody response induced by vaccinia virus; (ii) the production of polyclonal and...

Journal: :The Journal of antibiotics 1991
Y Ishii M Ichikawa K Yamaguchi K Takano M Inoue

Enterobacter cloacae NUH10 was isolated at Nagasaki University Hospital in 1987. E. cloacae NUH10 is a mutant strain which produces high levels of cephalosporinase. E. cloacae ATCC 23355 is known to be sensitive to so-called third generation cephems and produces an inducible cephalosporinase. The polyclonal antibody to cephalosporinase extracted from E. cloacae NUH10 was utilized in post-embedd...

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