JMH is a high-frequency human erythrocyte blood group antigen. Previous work has shown that JMH is absent from complement-sensitive erythrocytes of patients with paroxysmal nocturnal hemoglobinuria (PNH); such cells have a broad defect in expression of phosphatidylinositol (PI)-linked proteins. Using both human JMH antisera and a JMH-like murine monoclonal antibody, we have identified a 76-Kd m...

1. The agglutination of human erythrocytes and the precipitation of the blood group A substance by Type XIV antipneumococcus horse serum are properties of the specific anticarbohydrate immune bodies in the serum. 2. Absorption of Type XIV antipneumococcus horse serum with the homologous bacterial polysaccharide removes the agglutinins for human erythrocytes as well as the precipitins for the gr...

Erythrocytes are reported to release ATP in response to mechanical deformation and decreased oxygen tension. Previously we proposed that receptor-mediated activation of the heterotrimeric G protein G(s) resulted in ATP release from erythrocytes. Here we investigate the hypothesis that activation of heterotrimeric G proteins of the G(i) subtype are also involved in a signal transduction pathway ...

Human erythrocytes were suspended in Hank's solution containing mammalian or viral DNA or RNA. After dialysis at 0 degrees C first against water and subsequently against Hank's solution, and a further incubation at 37 degrees C, the erythrocytes were found to be loaded with the nucleic acids. The nucleic acid trapped in the erythrocytes exhibited up to 35 per cent of the external concentration.

I. Palekar AG, Tate SS, Meister A: Formation of 5-oxoproline from glutathione in erythrocytes by the -y-glutamyl transpeptidase cyclotransferase pathway. Proc NatI Acad Sci USA 71:293, 1974 2. Azzopardi 0, Jayle MF: Activite glutamyltransferase dans Ia membrane du globule rouge humain. Biochim Biophys Acta 389:339, 1975 3. Srivastava 5K, Awasthi YC, Miller SP, Yoshida A, Beutler E: Studies on -...

The aim of this study was to investigate the effect of clomazone herbicide on oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes in in vitro conditions. The activity of catalase (CAT), superoxide dismutase (SOD) and acetylcholinesterase (AChE), as well as the levels of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were measured in...

background: in recent years, the widespread use of microwave producing instruments specially mobile phones; result in growing concern regarding the possible effects associated with these waves on human health. in the present study investigated the genotoxic effects of mobile phone radiation in adult mice (balb/c) and their embryos. methods: in this experimental research pregnant mice were irrad...

Activation of the G protein G(s) results in increases in cAMP, a necessary step in the pathway for ATP release from rabbit and human erythrocytes. In all cells, the level of cAMP is the product of its synthesis by adenylyl cyclase and its hydrolysis by phosphodiesterases (PDEs). Both iloprost (Ilo), a PGI(2) analog, and isoproterenol (Iso), a beta-agonist, stimulate receptor-mediated increases ...

We used high-performance liquid chromatography with electrochemical detection to measure the catecholamine content of human erythrocytes. The ratio of free norepinephrine to epinephrine was three to four times lower than that in plasma and four to seven times lower than that in platelets. This suggests differences in uptake, degradation, or conjugation of norepinephrine and epinephrine by eryth...