نتایج جستجو برای: human antibody library

تعداد نتایج: 1863887  

Journal: :The new microbiologica 2012
Laura Solforosi Nicasio Mancini Filippo Canducci Nicola Clementi Giuseppe Andrea Sautto Roberta Antonia Diotti Massimo Clementi Roberto Burioni

A novel phagemid vector, named pCM, was optimized for the cloning and display of antibody fragment (Fab) libraries on the surface of filamentous phage. This vector contains two long DNA "stuffer" fragments for easier differentiation of the correctly cut forms of the vector. Moreover, in pCM the fragment at the heavy-chain cloning site contains an acid phosphatase-encoding gene allowing an easy ...

Hadi Nasiri, Jafar Majidi, Jalal Abdolalizadeh, Leili Aghebati-Maleki Mojghan Esparvarinha Tohid Kazemi, Zahra Valedkarimi

Antibodies are essential tools of biomedical and biochemical researches. Polyclonal antibodies are produced against different epitopes of antigens. Purified F(ab')2 can be used for animal’s immunization to produce polyclonal antibodies. Human immunoglobulin G (IgG) was purified by ion exchange chromatography method. In all stages verification method of the purified antibodies was sod...

Journal: :The Journal of biological chemistry 2000
P Steinberger J K Sutton C Rader M Elia C F Barbas

We describe the isolation of a CCR5-specific antibody, ST6, from an antibody phage display library generated from an immune rabbit. ST6 was previously shown to efficiently prevent the surface expression of CCR5 when expressed intracellularly (Steinberger, P., Andris-Widhopf, J., Buhler, B., Torbett, B. E., and Barbas, C. F., III (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 805-810). Because ST6 h...

2014
Aneta Schieferdecker Mareike Voigt Kristoffer Riecken Friederike Braig Thorsten Schinke Sonja Loges Carsten Bokemeyer Boris Fehse Mascha Binder

Bone homeostasis critically relies on the RANKL-RANK-OPG axis which can be targeted by the fully human monoclonal antibody denosumab in conditions with increased bone resporption such as bone metastases. The binding site and therefore the molecular mechanism by which this antibody inhibits RANKL has not been characterized so far. Here, we used random peptide phage display library screenings to ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2008
Chang C Liu Antha V Mack Meng-Lin Tsao Jeremy H Mills Hyun Soo Lee Hyeryun Choe Michael Farzan Peter G Schultz Vaughn V Smider

We have devised a phage display system in which an expanded genetic code is available for directed evolution. This system allows selection to yield proteins containing unnatural amino acids should such sequences functionally outperform ones containing only the 20 canonical amino acids. We have optimized this system for use with several unnatural amino acids and provide a demonstration of its ut...

Journal: :Bioscience reports 1988
I Rogers G D'Agostaro S Vera M Letarte

Monoclonal antibodies 50B4 and 50E6 recognize two distinct epitopes of human p85 glycoprotein (CDw44). Both epitopes are destroyed by reduction of the purified glycoprotein as demonstrated by inhibition of cellular radioimmunoassay and Western blot analysis. Endoglycosidase F treated p85 glycoprotein, with an apparent molecular weight of 73,000, is still reactive with both monoclonal antibodies...

2017
Ryan L. Kelly Jessie Zhao Doris Le K. Dane Wittrup

Efforts to develop effective antibody therapeutics are frequently hampered by issues such as aggregation and nonspecificity, often only detected in late stages of the development process. In this study, we used a high throughput cross-reactivity assay to select nonspecific clones from a naïve human repertoire scFv library displayed on the surface of yeast. Most antibody families were de-enriche...

2013
Michael M. Halford Maria L. Macheda Clare L. Parish Elena A. Takano Stephen Fox Daniel Layton Edouard Nice Steven A. Stacker

RYK is an unusual member of the receptor tyrosine kinase (RTK) family that is classified as a putative pseudokinase. RYK regulates fundamental biological processes including cell differentiation, migration and target selection, axon outgrowth and pathfinding by transducing signals across the plasma membrane in response to the high affinity binding of Wnt family ligands to its extracellular Wnt ...

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