Ex vivo expansion of hematopoietic stem cell (HSC) is an attractive technology for its potency of a variety of clinical applications. Such a technology has been achieved to some extent with combinations of various cytokines or continuous perfusion cultures. However, much more improvement is required especially for expansion of primitive hematopoietic progenitors. We propose here a novel molecul...

To construct an adenovirus vector co-expressing human bone morphogenetic protein (hBMP2) and human vascular endothelial growth factor (hVEGF165) as well as green fluorescence protein (GFP) as a marker, with which the intracellular expression of the inserted genes could be identified in Bone marrow mesenchymal stem cells (BM-MSCs). BMP2 and VEGF165 genes were PCR amplified from a cDNA library an...

To construct an adenovirus vector co-expressing human bone morphogenetic protein (hBMP2) and human vascular endothelial growth factor (hVEGF165) as well as green fluorescence protein (GFP) as a marker, with which the intracellular expression of the inserted genes could be identified in Bone marrow mesenchymal stem cells (BM-MSCs). BMP2 and VEGF165 genes were PCR amplified from a cDNA library an...

SUMMARY A malignant intraocular neoplasm that resembled human retinoblastomas was produced in CD rats by injection of human adenovirus type 12. Fluid (0.01 ml), containing adenovirus type 12, l0@@ 50% tissue culture infective doses/mI, from culture fluids ofhuman embryo kidney cells was injected into the vitreous cavity of each newborn rat. Three of 35 virus-injected rats developed an intraocul...

Tumor antigen in hamster tumors induced by adenovirus type 12 could be assorted by the complement-fixation test into two antigens : TS antigen, nonprecipitable by ultracentrifugation, and TP antigen, precipitable by ultracentrifugation. Tumor antigen was formed prior to the formation of infectious mature virus, viral coat protein, viral DNA or viral antigens in the lytic infection of human embr...

background and aims: adenovirus is one of the causative agents of viral conjunctivitis. approximately an average of 40% of viral conjunctivitis is due to adenovirus infection. the rate of infection is usually the highest during the spring and summer months. in this study attempt was made to evaluate the incidence of conjunctivitis due to adenovirus infection in patients referred to one of the a...

Prostate cancer (PCa) poses a high risk to older men and it is the second most common type of male malignant tumor in western developed countries. Additionally, there is a lack of effective therapies for PCa at advanced stages. Novel treatment strategies such as adenovirus‑mediated gene therapy and virotherapy involve the expression of a specific therapeutic gene to induce death in cancer cells...

CV787, a novel highly prostate-specific replication-competent adenovirus with improved efficacy, was constructed. CV787 contains the prostate-specific rat probasin promoter, driving the adenovirus type 5 (Ad5) E1A gene, and the human prostate-specific enhancer/promoter, driving the E1B gene. To improve efficacy, we constructed CV787 such that it also contains the entire Ad5 E3 region. CV787 rep...

Adenoviruses are potent vectors for inducing and boosting cellular immunity to encoded recombinant antigens. However, the widespread seroprevalence of neutralizing antibodies to common human adenovirus serotypes limits their use. Simian adenoviruses do not suffer from the same drawbacks. We have constructed a replication-deficient chimpanzee adenovirus-vectored vaccine expressing the conserved ...