The aim of the present study was to establish a protocol of sperm cryopreservation in Misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. We evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30 degrees C for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (dimethyl sulfoxide (DMSO)...

Introduction The brain is an astonishingly complex organ that records, responds and adapts to experience-all the result of simple interactions between neurons and glia. Whereas the neurons interconnect to form electrically active circuits, the role of glia is a mystery (Figure 1). Do glial cells just provide a passive framework that supports, nourishes, and insulates neurons, or, in addition, d...

The electrophysiological studies on the olfactory nervous system have been very few compared with those on the other sensory organs. It is well known that the fish can well smell out odorous substances in water. By observing the behaviour of fishes, Parker (1910, 1911) made it clear that the catfish and the killifish are able to smell odorous materials in water, and provided evidence that gusta...

-106and Xu, X.:Correlation between periodontal status and biting ability in Chinese adult population; Journal of Oral Rehabilitation, 30 (3): 260-264, 2003. 10)Han, L., Cruz, V. E., LI, M., Niwano, K., Abu-bakr, N., Okamoto, A., Honda, N. and Iwaku, M.:Effect of fluoride mouth rinse on fluoride releasing and recharging from aesthetic dental materials. Dent. Mater. J., 21(4):285-295, 2002. 11)Ko...

[3H]pseudomorphine (3H-PM) was prepared by the oxidation of [3H]morphine. A method for the estimation of 3H-PM in biological materials was developed with a minima1 sensitivity of 2.5 ng/ml of biologic material. Following the subcutaneous injection of 3H-PM (10 mg/kg as free base) in rats, the levels of 3H-PM in brain and spinal cord over a 2-hr period ranged between 46 to 17.9 rig/g and 24 to 7...

Five theoretical and/or experimental problems involving benzenoid polycyclics and related eystems are discussed. The dualiet graph characterizes uniquely any polycyclic aromatic hydrocarbon (PAH or pol.yhex), and differentiates between catafusenes, perifusenes and coronafusenes (catahexes, perihexes, coronahexes). 1. The topology of catafusenes, expre8sed by their dualist graphs, can be correla...

Mitosis-specific phosphorylation by cdc2 kinase causes nonmuscle caldesmon to dissociate from microfilaments during prometaphase. (Yamashiro, S., Y. Yamakita, R. Ishikawa, and F. Matsumura. 1990. Nature (Lond.). 344:675-678; Yamashiro, S., Y. Yamakita, H. Hosoya, and F. Matsumura. 1991. Nature (Lond.) 349:169-172). To explore the functions of caldesmon phosphorylation during cytokinesis, we hav...

Microtubule-associated proteins (MAPs) are the major accessory proteins on microtubules, the prominent, cylindrical component of the cytoskeleton. Despite the essential roles performed by microtubules in eukaryotic cells, little is known regarding the function of the filamentous MAPs, other that those that serve as motor proteins. Structural and biochemical analysis has revealed a great deal ab...

JCB • VOLUME 181 • NUMBER 1 • 2008 4 Spine actin for memory T iny spines on dendrites harbor three separate pools of actin, say Naoki Honkura, Haruo Kasai (University of Tokyo, Japan), and colleagues. The trapping of one pool helps form memory. Memory is thought to stem from long-term potentiation (LTP)—the lasting enhancement of communication between two neurons at a synapse. On the postsynapt...

published in Aduance ACS Abstracts, February 15, 1994. 0095-233819411634-0421$04.50/0 Fueled by the intense activity in fullerene chemistry and intrinsically interesting geometrical and topological features of fullerenes, several studies have focused on the fascinating mathematical properties of fullerenes. These studies included graph theoretical computations of the characteristic polynomials,...