Takagi, Shuichi, Yasuki Kihara, Shigetake Sasayama, and Tamotsu Mitsuiye. Slow inactivation of cardiac L-type Ca21 channel induced by cold acclimation of guinea pig. Am. J. Physiol. 274 (Regulatory Integrative Comp. Physiol. 43): R348–R356, 1998.—Whole cell L-type Ca21 current was recorded in ventricular myocytes dissociated from guinea pigs that were bred at ambient temperatures ranging betwee...

Abstract This work developed a fast and simple method for the quantification of two important ?-agonists, ractopamine (RAC) clenbuterol (CLEN) in animal urines. The was based on micro-extraction by mixed mode monolithic material analysis HPLC-UV UHPLC-Q Exactive TM Plus Orbitrap mass spectrometer. A functional monolith with nonpolar ester-based structure containing polar carboxylic groups therm...

In contrast to IVF, in ICSI the surrounding cumulus and corona cells must be removed completely, as only denuded oocytes can be successfully manipulated by the holding pipette. This ancillary effect of ICSI allows us to focus on the morphology of preimplantation development from the earliest stages. Early prognosis regarding the developmental fate of oocytes would help to limit a negative impac...

Voltage-dependent sodium channels from a variety of tissues are known to be phosphorylated by the cAMP-dependent protein kinase, protein kinase A. However, the functional significance of sodium channel phosphorylation is not clearly understood. Using whole-cell voltage-clamp techniques, we show that sodium currents (INaS) in rabbit cardiac myocytes are enhanced by isoproterenol (ISO). This enha...

Whole cell L-type Ca2+ current was recorded in ventricular myocytes dissociated from guinea pigs that were bred at ambient temperatures ranging between daily averages of 4 and 29°C. The dynamic voltage range of inactivation, as measured using 400-ms conditioning pulses and a holding potential of -40 mV, extended from -50 to -20 mV in myocytes prepared in summer. In winter, the inactivation curv...

The amplitude of the whole-cell L-type Ca2+ channel current recorded from vascular smooth muscle cells is reportedly greater in spontaneously hypertensive rats (SHR) than in Wistar-Kyoto rats (WKY). However, no study has examined properties of single Ca2+ channels in arterial cells from these strains. To further test the hypothesis that activation of L-type Ca2+ channels in arterial smooth musc...

Restitution of the conventional L-type calcium current (ICa) was studied in dog or guinea pig ventricular myocytes to understand its time course and regulation. Whole-cell ICa free of other overlapping currents was recorded with a suction pipette. The intracellular environment was varied by intracellular dialysis. The properties of ICa were similar in dog and guinea pig ventricular myocytes, ex...