The impact of Listeria monocytogenes listeriolysin O (LLO) secretion on phosphoinositide metabolism and mediator (platelet-activating factor and prostaglandin I2) generation was investigated in human umbilical vein endothelial cells. Wild-type L. monocytogenes, purified LLO, and an L. innocua strain engineered to secrete LLO all elicited a strong response, whereas mutant strains defective in LL...

*Javeed Iqbal,1 *Yulei Shen,1 *Yanyan Liu,1 Kai Fu,1 Elaine S. Jaffe,2 Cuiling Liu,1 Zhongfeng Liu,1 Cynthia M. Lachel,1 Karen Deffenbacher,1 Timothy C. Greiner,1 Julie M. Vose,3 Sharathkumar Bhagavathi,1 Louis M. Staudt,4 Lisa Rimsza,5 Andreas Rosenwald,6 German Ott,7 Jan Delabie,8 Elias Campo,9 Rita M. Braziel,10 James R. Cook,11 Raymond R. Tubbs,11 Randy D. Gascoyne,12 James O. Armitage,3 De...

AIM The present study was undertaken with the prime objective of isolating and identifying Listeria spp. from various foods of animal origin sold at retail market outlets in the city of Navsari, Gujarat. MATERIALS AND METHODS Total 200 samples comprising of milk, milk products, meat, and fish (50 each) collected aseptically from local market which were subjected first to pre-enrichment in hal...

Listeria monocytogenes, characterized by a high mortality rate in humans, is bacterium that causes listeriosis and found various aquatic products. The aim of this research was to investigate the presence, serotype distribution, virulence factor genes, antibiotic susceptibility L. monocytogenes strains isolated from total 500 fish samples whiting (Merlangius merlangus euxinus) (n:243) striped re...

Listeriolysin O, the major virulent determinant of Listeria monocytogenes, is known for forming pores on cholesterol-rich membranes. In the present study, we reveal its other facet, rafts clustering. By immunofluorescence microscopy, we show that the glycosylphosphatidylinositol-anchored proteins CD14 and CD24, which normally exhibit uniform distribution on J774 cells, undergo clustering upon t...

For rapid and inexpensive detection of polymerase chain reaction (PCR) amplicons, a novel microsphere agglutination assay has been developed. PCR is carried out using biotinylated forward and reverse primers, and the amplified DNA fragments are able to agglutinate streptavidin-coated microspheres (5.7 microm in diameter). Purification of PCR amplicons is unnecessary when initial primer concentr...

Screening for Asymptomatic Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis Infection using De-identified Urine Samples Received by the Navy Drug Screening Laboratory, San Diego Judith Harbertson, PhD, MPH; Paul Scott, MD, MPH; Paul Graf, PhD; Lisa Kennemur, PhD; Matthew Jamerson, PhD; Brent House, PhD; Melinda Balansay-Ames, BS; Chris A. Myers, PhD; Gary Brice, PhD; Brad...

The introduction into Brucella suis 1330 of a plasmid allowing the heterologous expression of a hybrid cytolysin containing listeriolysin from Listeria monocytogenes, and its export via the Escherichia coli hemolysin secretion pathway, resulted in secretion of active listeriolysin monitored by erythrocyte lysis. In contrast to observations with the nonhemolytic control strain, the phagosomes of...

Listeriolysine-O (LLO) is a 50KDa protein responsible for Listeria monocytogenes pathogenicity. The structure of LLO (PDB ID: 4CDB) with domains D1 to D4 is known. Therefore, it is of interest to identify conserved regions among LLO variants for destabilizing oligomerization (50 mer complex) of its monomers using appropriate inhibitors. Therefore, it is of interest to identify suitable inhibito...