DNA methylation is a key epigenetic mechanism that regulates gene expression and chromatin structure. This application note describes a workflow for analysis of DNA methylation in specific loci by methylationsensitive high-resolution melting (MS-HRM) followed by DNA sequencing of the reaction products (Figure 1). The workflow relies on Applied Biosystems® real-time PCR and capillary electrophor...

FIG. 1. Mutagenesis pipeline with time estimates. (A) Illustration of a Cas9-gRNA complex bound to target DNA. (B) Example of CRISPR target site design for six1a (NCBI Gene ID 494168), with the target sequence downstream of potential alternative start sites and upstream of the homeodomain. (C) Polymerase chain reaction products from a subset of injected embryos (green lines) are subjected to a ...

Melt copolymer chains are the main (most abundant) reaction product obtained when heating a blend of two (or more) condensation polymers (such as polyester + polycarbonate or polyester + polyamide or polyester + polyester) in which exchange reactions occur. In fact, during the melt-mixing reaction, an AB copolymer is formed and, as a consequence, the sample is a complex mixture made of three co...

[1] We derived the extent, onset date, end date, and duration of snowmelt in Antarctica from 1978 to 2004 using satellite passive microwave scanning multichannel microwave radiometer (SMMR) and Special Sensor Microwave Imager (SSM/I) data. A wavelettransform-based method was developed to determine and characterize melt occurrences. About 9–12% of the Antarctic surface experiences melt annually....

Introduction: Breccias are common among ordinary and enstatite chondrites [1]. Some type-3 ordinary chondrites have been described as primitive breccias because they contain fragments of types-3 to-6 chon-drites, carbonaceous, and impact-melted material [2]. Sahara 97072, one of the most primitive ECs [3], contains fragments of chondrules, shock-melt veins of Fe metal and troilite in eutectoid ...

Solenopsis invicta Buren (red imported fire ant) are invasive pests that have the capability of major destructive impacts on lifestyle, ecology and economy. Control of this species is dependent, in part, upon ability to estimate the potential spread from newly discovered nests. The potential for spread and the spread characteristics differ between monogyne and polygyne social forms. Prior to th...

High resolution melt (HRM) is gaining considerable popularity as a simple and robust method for genotyping sequence variants. However, accurate genotyping of an unknown sample for which a large number of possible variants may exist will require an automated HRM curve identification method capable of comparing unknowns against a large cohort of known sequence variants. Herein, we describe a new ...