results escherichia coli phytase was expressed in p. pastoris under different cultivation conditions (post-induction temperature, methanol concentration, and post-induction ph). the optimized conditions by rsm using face centered central composite design were 1% (v/v) methanol, ph = 5.8, and 24.5°c. under the optimized conditions, appa was successfully expressed in p. pastoris and the maximum p...

The process for the production of biodegradable plastic material (polyhydroxyalkanoates, PHAs) from microbial cells by mixed-bacterial cultivation using readily available waste (renewable resources) is the main consideration nowadays. These observations have shown impressive results typically under high carbon fraction, COD/N and COD/P (usually described as nutrient-limiting conditions) and war...

abstract in order to study of chemical and mechanical weed management methods and their integration in potato (solanum tuberosum), a field experiment was done in 2008 at ferdowsi university of mashhad research field. experimental design was completely randomize block design with 6 treatments and 3 replications. treatment included broadcast herbicide application (standard treatment), between row...

The aim of this study was to determine the effect differences addition culture medium concentrations on growth microalgae type Chlorella sp. Samples were obtained from Center for Brackish Water Cultivation Fisheries (BBPBAP), Jepara. This research conducted at Laboratory Nutrition and Natural Feed Sorong Marine Polytechnic with an experimental method using four treatments walne medium, there A ...

microalgal growth curve, after the exponential phase, shows a stationary phase where algalbiomass production is inhibited and remains constant by some factors such as nutrientdepletion and intrinsic behavior. the present study is concerned with evaluating effects ofthe culture medium condition and intrinsic behavior on biomass production by dunaliellatertiolecta. to this end, effect of ph, nutr...

Three kinds of phytoplankton were cultivated, and the contribution of dissolved organic matter (DOM) released from the phytoplankton was examined to clarify the cause of organic pollution of Lake Biwa. Microcystis aeruginosa, Staurastrum dorcidentiferum, and Cryptomonas ovata were evaluated with regard to cultivation. A significant peak (M(w): <3000 Da) was mainly detected in the algal DOM rele...

Copepods are considered relevant live feed for fish larval marine aquaculture. They promote survival and growth rate reduce deformities when used as food source. The cyclopoid copepod Apocyplops royi among others in extensive Taiwanese However, knowledge on its performance intensive cultures is limited. This study investigates parameters that prerequisites before initiating tank with A. royi. W...

Paraburkholderia sp. SOS3 is a plant growth-promoting bacterium (PGPB) that displays pleiotropic effects and has the potential to be applied at large scale across several agronomically important crops. The use of suitable option reduce chemical fertilisers. While benefits have been demonstrated in vitro, its applications are limited due low biomass yield current batch culture systems. Here, we ...

BACKGROUND Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen depletion, lack of pH control and the necessity to use low induction cell densities. In this article we describe the impact of a nove...