S,S'-bis(2-aminophenyl)ethanebis(thioate), (APhET), is reported as N2S2 ligand which form chelate with copper of high stability as compared to the other metals. Two modification of APhET, simpler 1,2-di-(o-aminophenylthio)ethane (DAPhTE), and the complex one 1,2-di-(o-salicylaldiminophenylthio)ethane (SAPhTE), were examined as the active material for copper(II) ion selective PVC membrane electr...

A sensitive and fully DNA-structured ion sensor was built by integrating polyT sequences for highly selective Hg2+ recognitions and two flanking G-quadruplex halves for allosteric signal transductions. The construction of this sensor was very easy that allowed a cost-effective detection of Hg2+ with a limit of detection of 4.5 nM, which was lower than the 10 nM toxic level for drinkable water a...

Recently, copper nanoclusters (CuNCs) have attracted great research interest for their low synthesis cost, wide application, and easy functionalization. Until now, CuNCs been developed applied in multi-fields such as sensing, catalysis, light-emitting diode manufacturing, cell imaging. Furthermore, the application of heavy metal ions (HMIs) detection is also regarded a major part fluorescence s...

A warning microanalyzer for the rapid monitoring of different heavy metals in water using Carbon Dots (CDs) as selective optical reagents is presented. The synthesized CDs have surface functionalization and exhibit fluorescence quenching by metal ions, that combined with use microfluidics, provide sensitivity, ease automation reproducibility to method. Moreover, they present maximum excitation ...

Raman spectra of ATP at various pH values are affected by addition of equimolar solution of divalent metal ions such as Ca2+, Mg2+, Co2+, Cu2+, and Hg2+. The changes in frequency and intensity have been used to construct models describing the nature of metal-adenine and metal-triphosphate interactions under different conditions. The metal ions are found to co-ordinate the triphosphate group in ...

A novel gene coding for an endo-beta-1,4-mannanase (manA) from Bacillus subtilis strain G1 was cloned and overexpressed in P. pastoris GS115, and the enzyme was purified and characterized. The manA gene consisted of an open reading frame of 1,092 nucleotides, encoding a 364-aa protein, with a predicted molecular mass of 41 kDa. The beta-mannanase showed an identity of 90.2-92.9% (< or =95%) wit...

N-Bromosuccinimide completely inactivated the cellulase, and titration experiments showed that oxidation of one tryptophan residue per cellulase molecule coincided with 100% inactivation. CM-cellulose protected the enzyme from inactivation by N-bromosuccinimide. The cellulase was inhibited by active benzyl halides, and reaction with 2-hydroxy-5-nitrobenzyl bromide resulted in the incorporation ...

In this study, a 39-kDa metalloprotease was purified from a rare edible mushroom with health-promoting activities, Oudemansiella radicata, using a purification protocol which entailed anion exchange chromatography on DEAE-cellulose and Q-Sepharose columns and gel filtration by fast protein liquid chromatography on a Superdex 75 column. Some peptide sequences were obtained by LC-MS/MS analysis a...

El presente trabajo tiene la finalidad de dar a conocer el desarrollo un prototipo sensor para medición mercurio (Hg2+) en solución acuosa sobre una plataforma electroquímica basada electrodos serigrafiados nanotubos carbonos (CNT-SPE) conjunto con nanopartículas oro (NPs-Au) realizadas través síntesis verde, utilizando extracto acuoso Sargassum spp. Para esto, se realizó caracterización las NP...