We have recently reported the cloning of a novel protein, TRiC-P5, with significant homology with protein 1 of the t-complex (TCP1). In the present study, the cellular localization of TRiC-P5 in Raji cells has been determined using an antiserum raised against a 18.5 kDa fusion protein. Results from cell fractionation and immunoblot studies indicate that TRiC-P5 is mainly localized in the cytopl...

We have identified Tim9, a new component of the TIM22.54 import machinery, which mediates transport of proteins into the inner membrane of mitochondria. Tim9, an essential protein of Saccharomyces cerevisiae, shares sequence similarity with Tim10 and Tim12. Tim9 is located in the mitochondrial intermembrane space and is organized into two distinct hetero-oligomeric assemblies with Tim10 and Tim...

Recent studies have shown that G-protein-coupled receptors (GPCRs) can assemble as high molecular weight homo- and hetero-oligomeric complexes. This can result in altered receptor-ligand binding, signaling, or intracellular trafficking. We have co-transfected HEK-293 cells with differentially epitope-tagged GPCRs from different subfamilies and determined whether oligomeric complexes were formed...

The heteroaggregation of engineered nanoparticles (ENPs) with natural colloids (NCs), which are ubiquitous in natural surface waters, is a crucial process affecting the environmental transport and fate of ENPs. Attachment efficiencies for heteroaggregation, α hetero, are required as input parameters in environmental fate models to predict ENP concentrations and contribute to ENP risk assessment...

Transmission electron microscope observation of mouse lymph nodes demonstrated that reticular fibers in the paracortex were invested not only by reticular cells, but occasionally also by migratory cells such as interdigitating cells, macrophages, and lymphocytes. Quantitative analysis of electron micrographs covering wide areas revealed that about 90% of the surface area of the reticular fiber ...

The relationship between the grinding characteristics of polymetallic complex ore and its component minerals, pyrrhotite, sphalerite, quartz, under action abrasion was studied, based on batch experiments theoretical analysis methods selective grinding. results show that when subjected to abrasion, crushing effect enhanced by existence is, sphalerite plays a positive role in ore. reduced pyrrhot...

Hetero-contacts are interfaces between different materials at the nanoscale leading to novel functional properties. In hetero-aggregates, primary particles of least two mixed particle or cluster level. Double flame spray pyrolysis (DFSP) is a versatile technique for controlled synthesis such materials. Characterization hetero-aggregates by scanning transmission electron microscopy (STEM) requir...

Transient processes of Ge/Si hetero-nanocrystal floating gate memories are simulated numerically. Compared with Si nanocrystal memories, Ge/Si hetero-nanocrystal memories show similar writing and erasing efficiency with a weaker writing saturation and markedly improved retention characteristics. 2006 Elsevier Ltd. All rights reserved. PACS: 72.20.Jv; 73.21.La; 73.90.+f; 74.50.+r

Plants optimise their resistance to herbivores by regulating deterrent responses on demand. Induction of anti-herbivory defences can occur directly in grazed plants or from emission of risk cues to the environment, which modifies interactions of adjacent plants with, for instance, their consumers. This study confirmed the induction of anti-herbivory responses by water-borne risk cues between ad...