Ticks are vectors for disease transmission because they are indiscriminant in their feeding on multiple vertebrate hosts, transmitting pathogens between their hosts. Identifying the hosts on which ticks have fed is important for disease prevention and intervention. We have previously shown that hemoglobin (Hb) remnants from a host on which a tick fed can be used to reveal the host's identity. F...

H UMAN FETAL HEMOGLOBIN consists of two unlike pairs of polypeptide chains, i.e., a2y2. While the detection of an a-chain variant may not present serious difficulties, since the abnormal fetal hemoglobin will be replaced by the adult type, the y-chain variants are transitory and their study is limited by time, shortage of blood and their rarity. Identification of the y-chain anomaly has been po...

Hemoglobinopathies are among the most common hereditary blood diseases worldwide and are considered a public health problem in some regions. In Brazil, hemoglobin S (Hb S) has a variable frequency between different regions mainly due to the ethnic composition of local populations. Due to the multiethnic characteristics of the Brazilian people, some regions reflect scenarios that allow us to con...

Hemoglobin (Hb) North Shore (beta 134 val leads to glu) is a mutant hemoglobin that is associated with the phenotype of mild heterozygous beta-thalassemia. Heterozygotes are characterized low normal hemoglobin levels or mild anemia, microcytosis, increased HbA2, and 34%-38% Hb North Shore. The mechanism of the anemia and microcytosis associated with Hb North Shore was explored by studies of hem...

By L. JUDEN REED, THOMAS B. BRADLEY, JR. AND HEu N M. RANNEY F ETAL HEMOGLOBIN, a tetramer of 2a and 2y polypeptide chains, constitutes the major hemoglobin component in newborn infants. During the first 6 months of extra-uterine life, the synthesis of y chains declines as chain synthesis increases. Adult Hb A, a tetramer of 2a and 2$ polypeptide chain accounts for over 95 per cent of the hemog...

We describe here a hemoglobin adduct assay applied to an analysis of samples from smokers and nonsmokers. The assay includes a sensitive method for quantification of orthotoluidine 2-aminonaphthylene, and 3- and 4-aminobiphenyl hemoglobin adducts in human blood using capillary gas chromatography-tandem mass spectrometry. Basic hydrolysis and derivatization with pentafluoropropionic acid anhydri...

Background: Serial blood draws for the assessment of a trauma patient’s hemoglobin (sHgb) and hematocrit (sHct) is standard practice. In the event of multiple casualties this process can be time consuming and lead to the inefficient use of valuable resources. A device that would allow for continuous real-time, non-invasive monitoring of hemoglobin and tissue perfusion would not only improve the...

Background: Various studies have come to contradictory results regarding the relationship between maternal hemoglobin (Hb) level and adverse pregnancy outcomes. The present study aimed to investigate the birth outcomes among pregnant women who had normal hemoglobin levels in the first trimester of their pregnancy. Patients and Methods: In this prospective study, 108 healthy pregnant women wi...

Precise oxygen equilibrium curves of hemoglobin (Hb) Chesapeake and human adult hemoglobin (Hb A) were determined by the automatic recording method in the presence and absence of NaCl, 2,3diphosphoglycerate (DPG), and inositol hexaphosphate (IHP). The Adair constants, kl, k2, k3, and kq, and some oxygenation parameters were obtained from the equilibrium data. Maximum slope of the Hill plot for ...

In the present study, we fabricated a hemoglobin/gold nanoparticle (Hb/GNP) heterolayer immobilized on the Au micro-gap to confirm H₂O₂ detection with a signal-enhancement effect. The hemoglobin which contained the heme group catalyzed the reduction of H₂O₂. To facilitate the electron transfer between hemoglobin and Au micro-gap electrode, a gold nanoparticle was introduced. The Au micro-gap el...