The present work studied the proliferation of epithelial cells when they were cultivated on substrates with micropillars fabricated with the same height but with different curvature characteristics. A special micro-fabrication method was employed to produce these micropillar substrates. Polyallyldiglycol carbonate (PADC) films were first irradiated by alpha particles and then chemically etched ...

Clonogenic survival of HeLa, Chinese hamster and HaK cells after treatment with a range of cisplatin concentrations and exposure times was determined and cellular platinum concentrations were measured by PIXE. It was demonstrated that cisplatin cytotoxicity of the three cell lines varied considerably as a function of drug exposure dose. These differences are related to differential cellular dru...

Cells. HeLa cells (American Type Culture Collection, Rockville, Md.) were grown in 80 ml culture medium in Roux bottles. Cells were grown in Eagle's medium (7) which contained Hanks' balanced salt solution (1 1), 10% bovine serum, 100 @zg/mlstreptomycin sulfate, and 100 units/ml penicillin G. Bottles were incubated at 36°in an humidified atmosphere of 5% CO2 , and the cells were passed by tryp...

Background & Aim:Cervical cancer is the most common   female genital cancer and the second most common cancer in women in the   world. Since the herbal ingredients have been used for centuries to treat   cancer, purpose of the present study was to determine the cytotoxic   effect of hydroalcoholic extract from Satureja bachtiarica Bungeon   Hela cancer cells line. ...

Two non-transformed revertants of the HeLa (ATCC CCL2) cell line have been isolated using a selection procedure based on prolonged retention of the fluorescent dye, rhodamine 123, within the mitochondria of carcinoma cells versus normal epithelial cells. Unlike the parental HeLa, the revertant cells exhibited a flat non-refractile morphology, failed to grow in suspension culture, had more than ...

objective: to study the mechanisms involved in amyloid formation processes, we made a mammalian cell culture model of amylin aggregation and characterized its properties. materials and methods: amyloid fibrils were extracted from cho cells and binding affinity to thioflavin t and congo red was investigated. then the apple-green birefringence of extracted fibrils was detected under polarized li...

Metabolic oligosaccharide engineering has been employed to introduce fluorine-containing groups onto mammalian cell surfaces. Incubation of HeLa, Jurkat, and HL60 cells in culture with fluorinated sialic acid and mannosamine analogues resulted in cell-surface presentation of fluorinated glycans. Metabolic conversion of fluorinated precursors was detected and quantified by DMB-derivatization and...

The naturally occurring polyphenol resveratrol (3,4',5-trihydroxy-stilbene, 3,4',5-THS, RES) has been shown as a chemopreventive and proapototic agent. Resveratrol is extensively metabolized by CYP450 enzymes. The monohydroxylation of resveratrol is catalyzed by CYP1B1 to form 3,3',4',5-THS (piceatannol), a metabolite with higher anticancer activity and stronger antioxidant properties. It was h...

The interactions of group B streptococci (GBS) with HeLa cells (an epithelial cell line) and MRC-5 cells (a fibroblastic cell line) were explored. A host-cell invasion assay using GBS strains from all serotypes revealed that GBS invaded HeLa cells to a greater extent than MRC-5 cells. One strain, a serotype V (NCS13), was highly invasive against HeLa cells. All strains were poorly invasive agai...

(1 to 7 mM). For cell extracts from all 3 cell lines, specific activities for the low-K^ phosphodiesterase are similar, although there is slightly higher activity in extracts of the fastest-growing Novikoff cells than in extracts from the other cell lines. In contrast, the specific activity for the high-Km phosphodiesterase is 30 to 40 times higher in L than in Novikoff cell extracts and interm...