Conventionally prepared immune serum globulin frequently produces severe side effects when administered intravenously. A modified preparation in which 4 to 5 interchain disulfide bonds have been reduced and alkylated has been made for intravenous use. However, reduction and alkylation may affect Fc-mediated functions of immunoglobulin G, particularly its ability to fix complement by the classic...

Damage arising from putative environmental sources has been found in the DNA of the gastric and colorectal mucosae of patients presenting with gastrointestinal disorders from the South Manchester area. 06-Methylguanine (06-MeG) in the range 0.010->0.300timolesmole-' adenine was heterogeneously distributed both between and within individuals. The pattern of alkylation of tissue DNA appears to di...

We have reductively alkylated deoxynojirimycin imino sugars using sodium cyanoborohydride to provide an efficient means of generating a series of N-alkylated compounds containing 4-18 carbon side chains. The yields were greater than 90% using a variety of aldehydes of different chain lengths, and after purification were >95% pure using (1)H-NMR. Radiolabelled compounds were prepared using sodiu...

Bacillus subtilis glutamine synthetase readily reacts with iodoacetamide, producing very specific changes in the catalytic parameters characteristic of the native enzyme. The alkylation reaction is influenced both by substrates and feedback inhibitors of the enzyme; whereas ammonia and glutamate are without effect, Mg2+-ATP enhances the alkylation reaction, and Mn2f-ATP effectively prevents the...

The steam cracker unit at ORLEN Unipetrol is a significant European producer of monomers. Due to heavy feedstocks, the can produce (besides ethylene, propylene and benzene) very valuable hydrocarbons such as dicyclopentadiene. staff (formerly Chemopetrol) has been investigating possibility producing dicyclopentadiene for almost 40 years. As result many years research activities several generati...

Analysis of the N-glycosylation pattern on antibodies is described using complementary mass spectrometric strategies based on both, top-down ESI-UHR-TOF and bottom-up LC-MALDI-TOF/TOF. Fast LC-ESI-UHR-TOF analysis, performed on the Bruker maXisTM, provides highresolution, high-mass accuracy (confident low ppm) data for both, intact antibodies and released antibody heavy chains allowing a rapid ...

RATIONALE Recent advances in analytical techniques have led to the development of gas chromatography/triple quadrupole mass spectrometry methods that allow the identification of target analytes in complex environmental samples. We have employed this technology to develop a method for characterizing alkylated chrysenes, which are environmental toxins that are resistant to weathering. METHODS A...