The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity. Using deletion constructs expressed as fusion proteins in E. coli, we localized this activity to the conserved homeodomain (HD). The binding site consensus, TCAATTAAAT, is found in clusters in the engrailed regulatory region. Weak binding of the En HD to one copy of a synthetic consensus is enhanced ...

We examined the immunogenicity and induction of inhibitory activity of 19-mer synthetic peptides which contained putative catalytic regions that were associated with the beta5 (EAW) and beta7 (HDS) strand elements of the suggested (beta,alpha)8 catalytic barrel domain of Streptococcus mutans glucosyltransferase (GTF). Both peptides readily induced serum immunoglobulin G (IgG) and salivary IgA a...

Despite varied etiologies and symptoms, several neurodegenerative diseases-specifically, Alzheimer's (AD), Parkinson's (PD), and Huntington's diseases (HDs)-share the common feature of abnormal circadian rhythms, such as those in behavior (e.g., disrupted sleep/wake cycles), physiological processes (e.g., diminished hormone release) and biochemical activities (e.g., antioxidant production). Cir...

The ion exchange intercalation of two Gd-based magnetic resonance imaging contrast agents into hydroxy double salts (HDSs) is reported. The presence of Gd(3+) diethylenetriaminepentaacetate and Gd(3+) diethylenetriaminepenta(methylenephosphonate) complexes in the HDS lattice after intercalation was confirmed by microwave plasma-atomic emission spectroscopy. The structural aspects of the HDS-Gd ...

This study evaluates the recovery of heavy metals employing a spent catalyst from the hydrodesulfurization (HDS) of diesel, with no chemical, thermal or physical pretreatment, using the bacterial strain Acidithiobacillus thiooxidans FG-01. Direct and indirect bioleaching tests were carried out in Erlenmeyer flasks (500 mL). The influence of the pulp density and supplementation with elemental su...

A key application of clustering data obtained from sources such as microarray, protein mass spectroscopy, and phylogenetic profile is the detection of functionally related genes. Typically, only a small number of functionally related genes cluster into one or more groups, and the rest need to be pruned. For such situations, we present Automated Hierarchical Density Shaving (Auto-HDS), a framewo...