A specific and sensitive radioimmunoassay for human choriogonadotropin (hCG) has been developed using rabbit antiserum to chemical analogs of beta subunit of human chorionic gonadotropin prepared by controlled reduction and S-alkylation of its disulfide linkages. The assay was highly specific for hCG as the binding of [125I]-hCG to the antibody was not affected by standard human lutropin, by hu...

The nanogold reaction between HAuCl4 and citrate is very slow, and the catalyst graphene oxide nanoribbon (GONR) enhanced the nanoreaction greatly to produce gold nanoparticles (AuNPs) that exhibited strong surface plasmon resonance (SPR) absorption (Abs) at 550 nm and resonance Rayleigh scattering (RRS) at 550 nm. Upon addition of the peptide of human chorionic gonadotropin (hCG), the peptide ...

We propose a novel hollow-core slow light waveguide using high contrast grating (HCG). Light propagates in air along a path bounded by two HCG layers. A strong interaction between the light and the HCG leads to a large group index, and thus the slow light effect. Waveguide loss and group index can be optimized separately by tuning the HCG and waveguide parameters. High performance slow light is...

This study was undertaken to determine which biological site or immunological site in the HCG molecule is essential for uptake of HCG by the ovaries. HCG was obtained from the urine of patients with trophoblastic dieseases by adsorption on kaolin. This preparation gave two distinct immunological fractions on Sephadex G-100 column chromatography. One fraction (antigen-1; Ag-1) had both gonadotro...

Increased serum levels of human chorionic gonadotropin beta subunit (hCG beta) were described previously in patients with bladder cancer. To obtain insight into such production of hCG beta, the expression of hCG beta 7, 8, 5, and 3 genes in bladder carcinomas and normal urothelia was investigated by reverse transcription PCR. Surprisingly, hCG beta mRNAs were detected in both normal urothelial ...

Human chorionic gonadotropin (hCG) is a heterogeneous molecule that is the marker of choice for detecting pregnancy and indicating ectopic pregnancies and spontaneous abortions. This article describes the use of hCG in detecting pregnancy, and how pregnancy detection is complicated by the extreme variation in individual hCG results and by the varying structure of hCG. The problems of false-posi...

Background: The -subunit of human chorionic gonadotropin (hCG) is encoded by four genes, of which expression of the hCG -3, -5, and -8 genes could have prognostic value in breast cancer. Methods: Applying a new, modified Molecular Beacon reverse transcription-PCR assay, we investigated the prognostic value of the hCG -3, -5, and -8 gene transcripts in 129 sporadic unilateral breast cancer sampl...

Chloride permeability pathways and progesterone (P4) secretion elicited by human chorionic gonadotropin (hCG) in human granulosa cells were studied by electrophysiological techniques and single-cell volume, membrane potential and Ca2+i measurements. Reduction in extracellular Cl(-) and equimolar substitution by the membrane-impermeant anions glutamate or gluconate significantly increased hCG-st...

The ability of human choriogonadotropin (hCG) to regulate its receptors and target cell responses has been studied in a clonal strain of cultured Leydig tumor cells (MA-10). Exposure of the MA-10 cells to hCG results in decrease in hCG binding activity which is dependent on time and the concentration of hCG. This decrease is due to a change in the number of receptors rather than in the affinity...