نتایج جستجو برای: h atpase غشای پلاسمایی
تعداد نتایج: 562717 فیلتر نتایج به سال:
در این پایان نامه مراحل ساخت و عملکرد غشای نانوفیلتر به منظور جداسازی پپسین (pepsin) مورد ارزیابی قرار گرفته است . در ابتدا غشای نامتقارن نانو فیلتراسیون pva/pes ساخته شد به نحوی که غشای pes به عنوان لایه اولیه و محافظ بر پایه ?? درصد وزنی پلیمر و به روش وارونگی فازها ساخته شد و سپس لایه کامپوزیتی از پلیمر pva با درصدهای وزنی مختلف روی آن پوشش داده شد که برای این کار کراس لینکر گلوترآلدهید اسید...
The gastric H+/K(+)-ATPase is a P-type ATPase that is specifically expressed in gastric parietal cells and is responsible for acid secretion into the stomach. We have found one or more gastric mucosal nuclear proteins that recognize a sequence motif in the 5'-upstream regions of the H+/K(+)-ATPase alpha- and beta-subunit genes. This gastric motif, (G/C)PuPu(G/C)NGAT(A/T)PuPy, may be a binding s...
Experiments from other laboratories conducted with Leishmania donovani promastigote cells had earlier indicated that the plasma membrane Mg2+-ATPase of the parasite is an extrusion pump for H+. Taking advantage of the pellicular microtubular structure of the plasma membrane of the organism, we report procedures for obtaining sealed ghost and sealed everted vesicle of defined polarity. Rapid inf...
تنش شوری باعث کاهش تمامی شاخصه های رشد گیاه از جمله وزن تر و خشک گیاهی، محتوای کلروفیل، طول ساقه، شاخص سطح برگ، محتوای آب گیاهی و همچنین کاهش غلظت عناصر پتاسیم، کلسیم و سیلیسیم در گیاه شد و نفوذپذیری غشاءپلاسمایی و غلظت سدیم را افزایش داد که کلیه این عوامل در نهایت منجر به کاهش عملکرد ماده خشک گیاهی در واحد سطح گردید. کاربرد سیلیس در محلول غذایی باعث افزایش وزن تر و خشک گیاهی، محتوای کلروفیل، ط...
When Streptococcus faecalis was grown in the presence of protonophores , an ATPase activity of the membrane was increased at a pH below 8.0 but not at a pH above 8.0. Characteristics of this increased ATPase were identical to those of a proton-translocating ATPase (H+-ATPase) located on the membrane of normal cells. The cytoplasmic pH was regulated at 7.6 to 7.8 but was not regulated in the pre...
A 100-kDa protein that is a main component of the microsomal fraction from rabbit gastric mucosa is phosphorylated by cAMP-dependent protein kinase (PKA) in the presence of 0.2% Triton X-100. Microsomes from rabbit gastric mucosa possess activity of H,K-ATPase but not activity of Na,K-ATPase. Incubation of microsomes with 5 microM fluorescein 5'-isothiocyanate (FITC) results in both an inhibiti...
ATPase activity in permeabilized cells of Helicobacter pylori as well as those of Helicobacter felis and Campylobacter jejuni was analyzed. The ATPase activities in these cells were most susceptible to sodium azide, fluoroaluminate, and dicyclohexylcarbodiimide, which are typical inhibitors of F ATPases. Optimal values for maximal activity were found to be at approximately pH 6.4, 6.0, and 6.0 ...
The vacuolar H(+)-ATPase (V-ATPase) has long been appreciated to function as an electrogenic H(+) pump. By altering the pH of intracellular compartments, the V-ATPase dictates enzyme activity, governs the dissociation of ligands from receptors and promotes the coupled transport of substrates across membranes, a role often aided by the generation of a transmembrane electrical potential. In tissu...
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