A simple, precise and rapid RP-HPLC method was developed for the determination of doxazosin mesylate in pharmaceutical formulations. The method was carried out on a Chromolith RP-C(18) column using a mixture of potassium phosphate buffer and methanol (40:60 v/v) and detection was done at 251 nm. The linearity range was 1-5 μg/ml. The retention time of the drug was 3.8 min. The LOD and LOQ were ...

A methodology of phosphopeptide-selective analysis coupled with column-switching HPLC utilizing titania as precolumn media is presented. Phosphopeptides were selectively enriched on titania packing within a protein/peptide mixture without any additional procedure, and analyzed by column-switching high-performance liquid chromatography. First, phospho-compounds were separated from complex mixtur...

Estimation of related substancesby usinghigh-performance liquid chromatographic method was developed and validated for the determination of Tigecycline in the present work. Reversedphase chromatography was performed on Waters 2489 UV 2695 pump, Waters 2998 PDA 2695 pump Software Empower2 photodiode array detector using Zorbax Eclipse plus C18 (100 mm × 4.6 mm, 1.8 μm particle size) column with ...