In order to develop antimicrobial mixtures which provide broad-spectrum antimicrobial activity for use with cultured cell autografts, several individual antimicrobial agents, in concentrations non-toxic for cells in culture, were tested against a variety of bacteria and Candida spp. isolated from burn patients. An agar well diffusion topical assay was used. Antimicrobials active against Gram-po...

In contrast to Gram-negative bacteria, secretory proteins of Gram-positive bacteria only need to traverse a single membrane to enter the extracellular environment. For this reason, Gram-positive bacteria (e.g. various Bacillus species) are often used in industry for the commercial production of extracellular proteins that can be produced in yields of several grams per liter culture medium. The ...

In the present study, we developed an assay of interactions of daptomycin with four model lipid membranes that mimicked mammal cell membranes and gram-positive and negative bacteria membranes. We also analyzed the binding kinetics of the gram-positive bacteria membranes using surface plasmon resonance (SPR). Daptomycin showed a higher affinity for the model gram-positive bacteria membrane than ...

The antibacterial activity of ethanol extract of Zingiber officinale Roscoe (ginger) and its combination with different salts like CuSO4, ZnSO4 and MnCl2 was investigated. Both Gram positive and Gram negative bacteria were tested by agar diffusion method. The results showed that ethanol extract of Zingiber officinale gave the maximum zone of inhibition at 50mg/ml concentrations against Escheric...

Most of our knowledge about pollutants and the way they are biodegraded in the environment has previously been shaped by laboratory studies using hydrocarbon-degrading bacterial strains isolated from polluted sites. In present study Gram-positive (Mycobacterium sp. IBBPo1, Oerskovia sp. IBBPo2, Corynebacterium sp. IBBPo3) and Gram-negative (Chryseomonas sp. IBBPo7, Pseudomonas sp. IBBPo10, Burk...

Opioid drug abusers have a greater susceptibility to gram positive (Gram (+)) bacterial infections. However, the mechanism underlying opioid modulation of Gram (+) versus Gram (-) bacterial clearance has not been investigated. In this study, we show that opioid treatment resulted in reduced phagocytosis of Gram (+), when compared to Gram (-) bacteria. We further established that LPS priming of ...

background and objective: antibiotic resistance is increasing, especially in healthcare-associated infections causing significant public health concerns worldwide. national information is required to make appropriate policies, update list of essential drugs for treatment, and evaluate the effects of intervention strategies. a nationwide surveillance of antimicrobial resistant bacteria in nosoco...

conclusions preliminary results indicate that the native iranian actinobacteria could be considered a suitable option for screening of the new antibacterial compounds. molecular research and antibacterial compound extraction against the aforementioned pathogenic strains are also being conducted. results based on the morphological characterization, 35 isolated cases belonged to actinomycetes and...

The insertion and the permanence of central venous catheters (CVC) represent potential sources of infection contracted in hospital. The evaluation of the risk of CVC-associated infections was evaluated in a retrospective study during the period 2007-2010 in a Hospital of Central Italy. A total of 514 CVC were collected and examined by microbiological techniques and, among the examined patients,...

Objective: Due to the alarming increase the incidence and prevalence of infectious diseases and increasing bacterial resistance to antibiotics need to discover new strong antimicrobial compounds is feeling.Methods: In this study, the antimicrobial properties of spider silk Pholcus phalangioides produced in sterile conditions against two bacteria, Listeria monocytogenes and <em...