mono-specific antisera against vibrio cholera ogawa nih-43 and vibrio cholera inaba 35-a3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. to visualize antigen-antibody reaction, gamma globulins were conjugated to staphylococcus aureus cowan-l (nctc: 8325) in th...

Although glycerol is the primary carbon source available to halophilic heterotrophic communities, little is known regarding haloarchaeal glycerol metabolism. In this study, a gene encoding a glycerol kinase homolog (glpK; HVO_1541) was deleted from the genome of the haloarchaeon Haloferax volcanii by a markerless knockout strategy. The glpK mutant, KS4, readily grew on yeast extract-peptone com...

background and objectives: preparation and evaluation of nanocomposite films have become prevalent in recent years. depending on the purpose of using the film, and considering the effects and interaction effects of nanomaterial and other modifiers on the film properties, the amounts of nanomaterials and modifiers should be optimized. the effects of montmorillonite (mmt), glycerol (gl), and thei...

introduction: biofilm forming staphylococcus epidermidis is a main causative agent of infections related to medical devices. purification and evaluation of gly-ta polysaccharide from a biofilm-forming s. epidermidis as a putative vaccine candidate were the main goals of the current study. methods: taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (gly-ta) was p...

Abstract Pigmentary coloration can produce viewing angle-independent uniform colors via light absorption by chromophores. However, due to the limited diversity in changes of molecular configuration chromophores undergo color change, existing materials cannot a wide range visible with tunable saturation and transmittance. Herein, we propose novel strategy create highly We fabricated hydrogel pol...

The 2-mono and 2,6-disubstituted methyl-1,4-dihydropyridines were synthesized by reaction of morpholine, thiophenol, 8-hydroxyquinoline, 2-naphthol and 2-mercopto-1-methyl imidazole with the 2-mono bromo and 2,6-dibromo-1,4-dihidropyridines.

Escherichia coli and Salmonella typhimurium show positive chemotaxis to glycerol, a chemical previously reported to be a repellent for E. coli. The threshold of the attractant response in both species was 10(-6) M glycerol. Glycerol chemotaxis was energy dependent and coincident with an increase in membrane potential. Metabolism of glycerol was required for chemotaxis, and when lactate was pres...

The oxidation and turnover of plasma glycerol has been studied in lean and obese, fed and starving man by means of a long-term infusion of glycerol-(14)C, and the participation of glycerol in gluconeogenesis has been determined. Under none of the experimental conditions did glycerol contribute more than 10% of the total respiratory CO(2). Glycerol turnover in fed lean subjects was 106 mmoles/mi...

Rainbow smelt is a small fish that accumulates glycerol in winter as a cryoprotectant when the animal is in seawater. Glycerol is synthesized in liver from different substrates that all lead to the formation of glycerol-3-phosphate (G3P). This study assesses whether glycerol is produced by a direct dephosphorylation of G3P by a phosphatase (G3Pase) or by a cycling through the glycerolipid pool ...

We report, in this work, our study of the thermal conductivity high-viscosity nanofluids based on glycerol. Three have been prepared with different contrasts, by suspending graphene flakes, copper oxides, or silica nanoparticles pure The were thermally characterized at room temperature 3ω technique, low amplitudes oscillations. A significant enhancement is found both glycerol/copper oxide and g...