Medicines of plant origin containing polysaccharides are used in pharmaceutical practice since they exhibit a wide spectrum thepharmacological activity. Species the Alchemilla L. genus Rosaceae family important scientific and practical importance; contain different groups biologically active substances (BAS), including phenolic compounds polysaccharides. The lack information literature on quant...

To characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae Hxt...

The nucleotides present in the cambial tissue (primary wall tissue) and in the not yet fully differentiated secondary xylem (secondary wall tissue) of Larix decidua Mill. were extracted and characterized. The method of extraction best suited to the material was investigated and the problems involved in desalting of extracts and their effect on the final nucleotide pattern obtained are discussed...

The human mammary gland is capable of de novo synthesis of glucose and galactose (hexoneogenesis); however, the carbon source is incompletely understood. In this study, we investigated the role of acetate, glutamine, lactate and glycerol as potential carbon sources for hexoneogenesis. Healthy breastfeeding women were studied following a 24-h fast on two occasions separated by 1-3 wk. Five women...

Regeneration of sugar nucleotides is a critical step in the biosynthetic pathway for the formation of oligosaccharides. To alleviate the difficulties in the production of sugar nucleotides, we have developed a method to produce uridine diphosphate galactose (UDP-galactose). The combined biosynthetic pathway, which involves seven enzymes, is composed of three parts: i) the main pathway to form U...

The deacylation of glucose, galactose and mannose pentaacetates, galactose and mannose penta(3-bromo)benzoates, as well as the dealkylation of 2,3,4,6-tetra-O-acetyl and 2,3,4,6-tetra-O-(3-bromo)benzoyl methyl alpha-D-glucopyranosides have been studied. In addition, a computational study on the deacylation of beta-D-glucose pentaacetate has been carried out with density functional theory (B3LYP...

Concentrated faecal extracts from breast and artificially fed infants during the first week of life were examined by paper chromatography. Besides lactose, glucose and galactose, five oligosaccharide spots were detected. Breast-fed infants showed an additional fucose spot. Complete hydrolysis of the individual oligosaccharides showed glucose and galactose always and fucose in two instances. The...

The metabolism of galactose compounds, especially that of uridine diphosphate n-galactose, has been studied in intact as well as in broken L cells and HeLa cells. It has been shown that the incorporation of n-galactose l-phosphate into UDP-galactose is rate-limiting in broken cell preparations, whereas the enzymatic 4-epimerization takes place at an appreciable rate. The latter reaction is stro...

The bound carbohydrates of noncataractous , decapsulated, human lenses have been examined. The hexoses and hexosamines of the water-soluble, water-insoluble nonmembrane, and membrane fractions of lenses from six age groups were determined by gas-liquid chromatography. The plasma membrane contains 60-70% of all bound carbohydrates, including glucose, galactose, mannose, fucose, glucosamine, and ...