The binary phase diagram of γ-gliadin, a wheat storage protein, in water was explored thanks to the microevaporator, an original PDMS microfluidic device. This usually qualified as insoluble aqueous environments, displayed partial solubility water. Two liquid phases, very dilute and dense phase, were identified after few hours accumulation time microevaporator. liquid–liquid separation (LLPS) f...

Exposure to gluten, a protein present in wheat rye and barley, is the major inducer for human Celiac Disease (CD), chronic autoimmune enteropathy. CD occurs about 1% worldwide population, genetically predisposed individuals bearing leukocyte antigen (HLA) DQ2/DQ8. Gut epithelial cell stress innate immune activation are responsible breaking oral tolerance gliadin, gluten component. To date, only...

Celiac Disease (CD) is an autoimmune disorder, represented by the ingestion of gliadin protein usually found in wheat, barley and rye. CD is closely associated with genes that code HLA-II antigens, mainly of DQ2 and DQ8 classes. Small intestinal biopsy is considered the gold standard for diagnosis of celiac disease. In addition, the most sensitive tests for the diagnosis of celiac disease inclu...

The utility of mining DNA sequence data to understand the structure and expression of cereal prolamin genes is demonstrated by the identification of a new class of wheat prolamins. This previously unrecognized wheat prolamin class, given the name δ-gliadins, is the most direct ortholog of barley γ3-hordeins. Phylogenetic analysis shows that the orthologous δ-gliadins and γ3-hordeins form a dist...

Vegetal proteins have emerged as appealing starting materials for the development of various drug delivery systems, and their use obtaining polymeric nanoparticles has been profitably exploited in multidisciplinary fields. Wheat gliadin, water-insoluble storage protein gluten, is characterized by a great amount hydrophobic amino acid residues notable mucoadhesive features. This biopolymer can b...

BACKGROUND An increased prevalence of coeliac disease in patients with primary biliary cirrhosis has been recently reported. However, in other studies the association has not been confirmed. There have been no formal attempts to systematically evaluate patients with autoimmune cholangitis for coeliac disease. METHODS Sera from 62 patients with primary biliary cirrhosis, 17 with autoimmune cho...

Celiac Disease (CD) is a chronic inflammatory enteropathy, triggered in genetically susceptible individuals by dietary gluten. Gluten is able to elicit proliferation of specific T cells and secretion of inflammatory cytokines in the small intestine. In this study we investigated the possibility that p10-mer, a decapeptide from durum wheat (QQPQDAVQPF), which was previously shown to prevent the ...

To assess whether dietary antigens play a role in inflammatory bowel disease, 26 monozygotic twin pairs with inflammatory bowel disease and 52 healthy controls were investigated for serum antibodies (IgA, IgG, IgM) against ovalbumin, betalactoglobulin, gliadin, whole yeast (Saccharomyces cerevisiae) and yeast cell wall mannan. The twins were made up of five pairs concordant and nine pairs disco...

IgA nephropathy is the most common form of primary glomerulonephritis worldwide. Mucosal infections and food antigens, including wheat gluten, have been proposed as potential contributing environmental factors. Increased immune reactivity to gluten and/or association with celiac disease, an autoimmune disorder triggered by ingestion of gluten, have been reported in IgA nephropathy. However, stu...