نتایج جستجو برای: giemsa staining method
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In this study, a detailed chromosome analysis of the endemic Cappadocian Chub, Squalius cappadocicus in Melendiz Stream (Aksaray) was performed. The standard Giemsa staining, C-banding (CBG and CB-DAPI), Ag-NOR technique were applied. diploid number 50; its karyotype formula 14M + 16Sm 10St 10A. Heteromorphic sex chromosomes weren’t detected studied specimens. all chromosomal arms (NF) 90. C-ba...
The object of this paper is to describe a simple method for the staining in smear preparations of Spiroch~eta pallida and to present certain observations which I have been enabled to make upon the morphology and reproduction of the microorganism through the use of this staining method. I have examined a large number of cases of syphilis and various syphi-litic lesions, and it is upon the study ...
background and purpose: microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. the prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. in the present study, we aimed to investigate microsporidial keratitis in patients referring to farabi eye hospital tehran, iran ...
Human metaphase chromosomes were photooxidized in the presence of methylene blue, a process that destroys guanine residues in DNA. Indirect immunofluorescence showed that such chromosomes reacted with a cytosine-specific antibody revealing a consistent fluorescent banding pattern by which each chromosome could be identified. The observed fluorescent patterns were the reverse of those produced i...
This research presents the unusual staining characteristic of teleosts red blood cells–the common bluestripe snapper Lutjanus kasmira, caught at Toc Tan (Allison) Reef, Truong Sa Archipelago, Vietnam, due to side effects delayed procedure. In addition quantitative data measured, results microscopic image showed that, on slides performed with Romanovsky procedure using Giemsa dye, cell "nucleus"...
A girl with a new de novo translocation of 4q onto the short arm of acrocentric 22 is reported as a case of "pure" partial trisomy 4q. Her karyotype was 46, XX,-22, +mar, t(4;22)(q25-->qter;p11) identified by Giemsa staining and FISH. Comparison of the proband with previously reported cases of "pure" partial trisomy 4q showed the main clinical features to be growth retardation, psychomotor reta...
Introduction: Considering the importance of co-culture in differentiation of embryonic stem cells, the aim of this study was evaluation of the effect of co-culturing fetal liver stroma cells with P19 cells on the line of differentiation. Materials and Methods: For this purpose, P19 cells were cultured directly in semisolid medium. These cells proliferated and primarily differentiated to col...
Fig. 1. (A–C) Features of the bone marrow (BM) aspirate and biopsy showing hemophagocytic lymphohistiocytosis (HLH) and cytomegalovirus (CMV) staining. (A, B) BM aspirate showing histiocytic hyperplasia and prominent hemophagocytosis by activated histiocytes. Arrowheads indicate features suggestive of ongoing endocytosis (Wright-Giemsa stain, ×1,000). (C) BM core biopsy result showing positive ...
equine piroplasmosis is a tick-borne disease caused by intra-erythrocyte protozoa, theileria equi and babesia caballi. the present study aimed to detect piroplasm infection in horses in kurdestan province, iran, through molecular and microscopic approaches.n this study, 186 blood samples were randomly collected from horses of five regions of kurdestan province. the tbs-s/tbs-a primer set was us...
The prevalence of cryptosporidial infection among captive wild animals and birds in Sanda Kyarimi Park, Maiduguri, north-eastern Nigeria, was evaluated by faecal examination for oocysts using three different staining techniques: the modified Ziehl-Neelsen, Giemsa, and Safranin-methylene blue. A total of 66 captive wild animals and birds were examined based on age and sex differences. Fifteen (2...
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