We screened a phage display peptide library for peptidyl mimotopes of gibberellin against anti-bioactive gibberellin antibody. The peptides obtained were grouped into two homologous sequences and their binding to the antibody was put in competition with free GA(4) but not with GA(4) methylester, suggesting that the peptides behave as mimics of GA(4). As an application, the phage display peptide...

(-)-Spirobrassinin, a glucosinolate compound from Brassicaceae plants, has shown inhibitory effects on weeds. However, its specific target sites are not well explored. This study used molecular docking, dynamics simulations, and biological experiments to investigate (-)-Spirobrassinin’s sites. The of (-)-Spirobrassinin were observed various enzymes crucial for plant metabolic pathways, includin...

Floral buds were induced either by 8 h photoperiods or by the application of GA3 and GA13 in Impatiens bahamina. Total enzyme activity was not directly related to floral morphogenesis although some interesting qualitative correlations existed. Thus, the presence of a new isoenzyme of amylase {Rp 0-05) may be related to flower formation since it was present only in the induced plants and not in ...

We developed abscinazole-E1 (Abz-E1), a specific inhibitor of abscisic acid (ABA) 8'-hydroxylase (CYP707A). This inhibitor was designed and synthesized as an enlarged analogue of uniconazole (UNI), a well-known plant growth retardant, which inhibits a gibberellin biosynthetic enzyme (ent-kaurene oxidase, CYP701A) as well as CYP707A. Our results showed that Abz-E1 functions as a potent inhibitor...

Glomus mosseae, a representative species of Endogonaceae (Phycomycetes) able to form vesicular-arbuscular mycorrhiza, was investigated for phytohormone production. Spores of G. mosseae were axenically germinated in water, and the resultant mycelial growth was assayed by standard procedures for extracting plant hormones from microbial cultures. Paper partition chromatography and specific bioassa...

Although the aleurone layer of ceral grain seeds has many advantages for the study of gibberellin action, it has the disadvantage that the hand-isolation of the aleurone layers is time-consuming. To overcome this disadvantage, a commercially available pasta machine was modified and used to remove aleurone layers from imbibed barley (Hordeum vulgare) seeds. This equipment allows isolation of a t...

transgenic and non transgenic nicotiana tabacum l (cultivar wisconsin) containing ri t-dna were treated with 0, 0.2 and 0.4 mgl-1 ga3 in murashig and skoog medium. some physiological parameters including shoot length, leaf area, number of auxiliary bud, fresh and dry weight, number and length of trichomes were measured. shoot length, fresh weight and dry weight were increased but number of tric...

Several compounds closely related to choline and of the structure CHoX-CHo-N+ (CH3)3 * Clare active as plant growth substances when X is a Cl, Br, or = CH2 group (14). Treated plants develop thicker stems, shorter internodes, and intensely dark green leaves (7, 16, 17). The growth changes simulate those caused by exposure of plants to high light intensities, a short photoperiod, and low tempera...