Cell polarization is a prerequisite for essential processes such as cell migration, proliferation or differentiation. The yeast Saccharomyces cerevisiae under control of the GTPase Cdc42 is able to polarize without the help of cytoskeletal structures and spatial cues through a pathway depending on its guanine nucleotide dissociation inhibitor (GDI) Rdi1. To develop a fundamental understanding o...

ER-to-Golgi transport in yeast may be reproduced in vitro with washed membranes, purified proteins (COPII, Uso1p and LMA1) and energy. COPII coated vesicles that have budded from the ER are freely diffusible but then dock to Golgi membranes upon the addition of Uso1p. LMA1 and Sec18p are required for vesicle fusion after Uso1p function. Here, we report that the docking reaction is sensitive to ...

Full adder circuit is an essential component for designing of various digital systems. It is used for different applications such as Digital signal processor, microcontroller, microprocessor and data processing units. Due to scaling trends and portability of electronic devices there is a high demand and need for low power and high speed digital circuits with small silicon area. So, design and a...

Chronic renal failure (CRF) patients are considered to present genomic instability and, as a consequence, elevated levels of genetic damage. An open question is whether this damage is related to the stage of the pathology. To determine the background levels of genetic damage, a large population of 258 Caucasian adults (201 CRF patients and 57 controls) was analysed using the micronucleus (MN) a...

The difficulty in assessing the cost output and outcome for Geospatial Data Infrastructures (GDI’s) can be traced back to the differences between the various models and techniques that are applied interchangeably. These include economic models, statistical techniques, responsibility accounting and value approaches. Various publications have described either estimates of the total cost, possible...

Results of damage prediction in buildings can be used as a useful tool for managing and decreasing seismic risk of earthquakes. In this study, damage spectrum and C4.5 decision tree algorithm were utilized for damage prediction in steel buildings during earthquakes. In order to prepare the damage spectrum, steel buildings were modeled as a single-degree-of-freedom (SDOF) system and time-history...

In damage detection the number of elements is generally more than the number of measured frequencies. Consequently, the corresponding damage detection equation is undetermined and thus has infinite solutions. Since in the damaged structures most of their elements remain healthy, the sparsest solution for the damage detection equation is mostly the actual damage. In the proposed method, the dama...

Invadopodia are specialized membrane protrusions composed of F-actin, actin regulators, signaling proteins, and a dynamically trafficked invadopodial membrane that drive cell invasion through basement membrane (BM) barriers in development and cancer. Due to the challenges of studying invasion in vivo, mechanisms controlling invadopodia formation in their native environments remain poorly unders...

As combustion can widely vary between engine cycles if left uncontrolled, strict and robust control methodologies required to meet optimum performance at different operating conditions. Although the direct injection stratified charge engine development attempts started since 1920, lack of advanced instrumentation and robust real-time control techniques restricted its developments to common usag...