Hajšlová J., Lancová K., Sehnalová M., Krplová A., Zachariášová M., Moravcová H., Nedělník J., Marková J., Ehrenbergerová J. (2007): Occurrence of trichothecene mycotoxins in cereals harvested in the Czech Republic. Czech J. Food. Sci., 25: 339–350. A large-scale survey of the natural occurrence of trichothecene mycotoxins in major cereals harvested in the Czech Republic was conducted during th...

Controls in food industry are fundamental to protect the consumer health. For products of high quality, warranty of origin and identity is required and analytical control is very important to prevent frauds. In this article, the "state of art" of mass spectrometry in enological chemistry as a consumer safety contribute is reported. Gas chromatography-mass spectrometry (GC/MS) and liquid-chromat...

An improved method is presented, based on gas chromatography-electron capture mass spectrometry (GC-EC-MS), for measuring N7-(2'-hydroxyethyl)guanine (N7-HEG) in DNA from an in vivo sample. The method was used to detect this adduct in amounts of human DNA ranging from 0.07 to 11.5 microg isolated from granulocytes. In this method, the DNA is spiked with a stable isotope internal standard (N7-HE...

The method for simultaneous determination of residues of seven pyrethroids (bifenthrin, cyfluthrin, lambda-cyhalothrin, cypermethrin, deltamethrin, fenvalerate and permethrin) in animal meat samples has been developed and validated. The method is based on the extraction of pyrethroid residues with mixture of acetone-petroleum ether, freezing step, clean-up on a Florisil column, and finally dete...

This gas-chromatographic method for assay of fluoxetine and norfluoxetine in human plasma involves extraction of the drugs and use of a 63Ni electron-capture detector. The linear range of detection is 25 to 800 micrograms/L for each drug. Overall precision (CV) in the concentration range of 10 to 100 micrograms/L for both drugs was approximately 10%. Accuracy (relative error) in the same concen...

The present assay was developed for quantifying midazolam in plasma of patients hospitalized in intensive-care units or undergoing anesthesia and receiving many other drugs as well. Plasma samples are alkalinized with NaOH and midazolam is extracted into n-hexane. The organic phase is evaporated and reconstituted in n-butyl acetate, and the midazolam is quantified by gas-liquid chromatography w...

A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal stand...

We describe a procedure for determing clorazepate dipotassium as its decarboxylated, pharmacologically active metabolite, desmethyldiazepam, in 100 microL of plasma, with use of electron-capture gas--liquid chromatography and with methylnitrazepam as the internal standard. The procedure is a one-tube, one-step extraction without derivative formation and is accurate, reproducible, and rapid. The...

An electron-capture negative chemical ionization (NCI) gas chromatography-mass spectrometry (GC-MS) method for determination of lead (Pb) in blood samples is described. Extraction of Pb from the sample does not involve hot digestion but is based on treatment at ambient temperature. The blood sample is supplemented with a known amount of internal standard (204Pb) for isotope dilution and is trea...

._----__-_--_-_-______--_----_--_-_----_--__--------_-Bl Introduction-__-____--_-_---_____---_--_---__-_--------_--------__ 1 Collection and handling of samples.-_-----_-__--__-------_-_---__--3 Electron-capture gas chromatographic method-___..---_---,______.___ 4 Apparatus.-__-__---_-----_------------_-------_-_-------__-__ 4 Reagents-_-____----_---______-_---_-_-_-_-_-_-___-_---_-----4 Proced...