When desialylated ceruloplasmin (ASCPN), labeled with 64Cu and with 3H in its galactose residues, is injected intravenously into a rat, more than half of the radioactivity can be recovered within minutes in ASCPN precipitated immunochemically from liver homogenates. Both radioautographic studies and measurements of hepatic uptake of ASCPN following blockade of the reticuloendothelial system ind...

When desialylated ceruloplasmin (ASCPN), labeled with 64Cu and with 3H in its galactose residues, is injected intravenously into a rat, more than half of the radioactivity can be recovered within minutes in ASCPN precipitated immunochemically from liver homogenates. Both radioautographic studies and measurements of hepatic uptake of ASCPN following blockade of the reticuloendothelial system ind...

A highly purified preparation of 1,2-oc-L-fucosidase, free of /3-galactosidase and P-N-acetylglucosaminidase activities, has been obtained from a commercial preparation of Aspergillus niger by a simple isolation procedure involving ammonium sulfate precipitation, pressure dialysis, repeated gel Cltration on Sephadex G-150, and chromatography on diethylaminoethyl Sephadex A-50. The enzyme has a ...

The non-enzymatic reaction between reducing sugars and proteins, known as glycation, has received increased attention from nutritional and medical research. In addition, there is a large interest in obtaining glycoconjugates of pure well-characterized oligosaccharides for biological research. In this study, glycation of bovine serum albumin (BSA) by d-glucose, d-galactose and d-lactose under dr...

Phosphocholine (PCho) is an important substituent of surface structures expressed by a number of bacterial pathogens. Its role in virulence has been investigated in several species, in which it has been shown to play a role in bacterial adhesion to mucosal surfaces, in resistance to antimicrobial peptides, or in sensitivity to complement-mediated killing. The lipopolysaccharide (LPS) structure ...

The carbohydrate moiety of HLA antigens was radioactively labeled in galactose residues by treatment of membranes with neuraminidase, galactose oxidase, and NaB”H,. A single N-linked oligosaccharide side chain M, 2700 to 3300 was labeled by this treatment. Unique pronase and tryptic “H-glycopeptides were purified, using lentil-lectin affinity chromatography. They contained all the radioactivity...

Type I collagen is the most abundant structural protein in vertebrates. It is a heterotrimeric molecule composed of two α1 chains and one α2 chain, forming a long uninterrupted triple helical structure with short non-triple helical telopeptides at both the N- and C-termini. During biosynthesis, collagen acquires a number of post-translational modifications, including lysine modifications, that ...

The saccharidic content of the glycoconjugates has been studied in the descended the undescended testes of a 8 years old boy. For this purpose, a battery of seven HRP-conjugated lectins (SBA, DBA,PNA,WGA,UEAI, LTA and ConA) was used. D-galactose-N-acetyl-D-galactosamine and alpha-L-fucose sugar residues, which were present in the cytoplasm of the Sertoli cells of the normally positioned prepube...

Background: Curcumin is the most active ingredient in turmeric root of Curcuma longa of the Zingiberaceae family and has a potent antioxidant activity. This study aimed at investigating the effects of curcumin with various doses on the density of dark neurons in the hippocampus of induced D-galactose aging mice model. This experimental study was conducted on 40 adult male BALB/c mice.  Materia...