Thousands of different kinds lichen metabolites are being examined for their biological activities, including anticancer properties. In this context, the present study aims to assess anti-inflammatory activity acetone extract Parmotrema austrosinense mycobiont. A lipid peroxidation assay was performed with extracts P. mycobiont, which further used evaluate its efficacy using a zebrafish model. ...

Canine distemper virus (CDV) creates a very contagious viral multi-systemic canine distemper (CD) disease that affects most species of Carnivora order. The virus is genetically heterogeneous, particularly in section of the hemagglutinin (H) gene. Sequence analysis of the H gene can be useful to investigate distinction of various lineages related to geographical distribution and CDV molecular ep...

Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...

Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...

Objectives: Royal jelly (RJ), a secretion of both the hypopharyngeal and mandibular glands of nurse workers, is believed to play a central role in honeybee queen development. Important component of royal jelly are proteins which form about 50% of the dry mass of RJ. Major royal jelly proteins (MRJPs) are the dominant proteinaceous component of royal jelly and constitute about 82-90% of total pr...

perforin gene (prf1) mutations have been reported in 20-30% of patients with familial hemophagocytic lymphohistiocytosis (fhl), an immune disorder of infancy and early childhood. cytotoxic t and natural killer (nk) cell activities are remarkably reduced or ab-sent in fhl patients. we report the first cases of familial hemophagocytic lymphohistiocy-tosis in an iranian family with two siblings. e...

diacylglycerol acyltransferase1 (dgat1) has been introduced as a strong potential candidate gene for milk fat content in cattle. dgat1 gene plays a key role in the final step of triglyceride synthesis. in this study, the genetic variability in the region spanning exon 1 to exon 2 of the buffalo dgat1 gene was investigated by dna sequencing. two hundred buffaloes belonging to three indigenous po...

Evolution of glyphosate-resistant kochia is a threat to no-till wheat-fallow and glyphosate-resistant (GR) cropping systems of the US Great Plains. The EPSPS (5-enol-pyruvylshikimate-3-phosphate synthase) gene amplification confers glyphosate resistance in the tested Kochia scoparia (L.) Schrad populations from Montana. Experiments were conducted in spring to fall 2014 (run 1) and summer 2014 t...

Abstract This study included 80 blood specimens. Fifty samples collected from COVID 19 with age ranged between 02-75 years, and 30 specimens healthy as a control sample 91-63 years. The polymorphism of IFN-γ T/A +874 gene, which amplified by using amplification refractory mutation system (ARMS-PCR) was showed high percentage A allele frequency in patients comparison T frequency, the revealed et...