The measurement of intracellular calcium response transients in living mammalian cells is a popular functional assay for identification of agonists and antagonists to receptors or channels of pharmacological interest. In recent years, advances in fluorescence-based detection techniques and automation technologies have facilitated the adaptation of this assay to 384-well microplate format high-t...

Objective(s): The current study reports the production and biocompatibility evaluation of a ternary nanocomposite consisting of HA, PLA, and gelatin for biomedical application.Materials and Methods: Hydroxyapatite nanopowder (HA: Ca10(PO4)6(OH)2) was produced by burning the bovine cortical bone within the temperature range of 350-450 oC followed by heating in an oven at 800. Synthesis of the te...

a series of 3-hydroxypyridin-4-one derivatives (hpos) were synthesized and their partition coefficient values (k part ) were determined. the cytotoxic effects of these iron chelators against hela cancer cells were also evaluated. the ic 50 of hpos was determined using mtt assay. among these ligands, compound 4e (k part =5.02) with an ic 50 of 30 µm and 4f (k part =0.1) with an ic 50 of 700 µm s...

Background: Vasopressin type 2 receptor (V2R) plays an important role in the water reabsorption in the kidney collecting ducts. V2R is a G protein coupled receptor (GPCR) and the triplet of amino acids aspartate-arginine-histidine (DRH) in this receptor might significantly influence its activity similar to other GPCR. However, the role of this motif has not been fully confirmed. Therefore, the ...

conclusions genome instability in addicted patients was demonstrated in this study. controversially, considering incoherent results of previous studies and our data, more studies in longer duration of methadone use are needed to elucidate the consequence. results the mutagenicity index in addicted group was robustly higher than healthy volunteers. fortunately, this significant difference was lo...

Of the explanations proposed for these differences by the various investigators, the lower recovery of the second-generation assay experienced by Garg et al. UK), from which can be calculated a positive bias and mean recovery of 114% (range, 102.1–121.7%) in 20 samples to which 3–28 ␮g/L tacroli-mus was added and a similar overestimate relative to the results reported by the small number of cen...

BACKGROUND & OBJECTIVE Mannose binding lectin (MBL), a C-type or Ca(2+) dependent lectin, plays a major role in lectin pathway of complement activation. MBL deficiency/insufficiency is associated with susceptibility to many infections. It is important to know the association of functional lectin levels with disease condition. Therefore, we carried out this study to develop a simple assay to est...

Departments of Genetics, Pathology, Radiation Oncology, Medical Oncology and Cancer Genomics Center Netherlands, Erasmus University Medical Center, Rotterdam, The Netherlands Department of Clinical Genetics, VU University Medical Center, Amsterdam, The Netherlands Division of Molecular Pathology, Netherlands Cancer Institute, Amsterdam, The Netherlands Departments of Toxicogenetics and Human Ge...

Abstract Background Dietary and wholesome antioxidant rutin is considered advantageous due to its potential protective role for numerous diseases related oxidative stress, high safety, cost-effectiveness, extensive biological effects. The present study accounts an expeditious method the synthesis of silver nanoparticles (AgNPs) using rutin. Results presence AgNPs was affirmed by UV–visible spec...

The recent completion of a working draft of the human genome sequence promises to provide unprecedented opportunities to explore the genetic basis of individual differences in complex behaviours and vulnerability to neuropsychiatric illness. Functional neuroimaging, because of its unique ability to assay information processing at the level of brain within individuals, provides a powerful approa...