Cryopreservation of seabass (Lates calcarifer) semen is an approach to increase the quantity and quality of seabass fry in aquaculture. However, cold shock can induce sperm injury leading to structural damage of the plasma membrane and loss of motility. Thus, the effect of cryopreservation on fatty acid composition and antioxidant enzyme activities of seabass sperm was determined. In cryopreser...

Cryopreservation of seabass (Lates calcarifer) semen is an approach to increase the quantity and quality of seabass fry in aquaculture. However, cold shock can induce sperm injury leading to structural damage of the plasma membrane and loss of motility. Thus, the effect of cryopreservation on fatty acid composition and antioxidant enzyme activities of seabass sperm was determined. In cryopreser...

The objective was to determine whether transfer of fresh or vitrified embryos produced in vitro with sex-sorted semen improves pregnancy and calving rates during summer in lactating dairy cows compared with artificial insemination (AI). Lactating dairy cows (n=722) were enrolled during summer months at 2 commercial dairies in Central Texas and randomly assigned to 1 of 3 treatments: AI with con...

Timed artificial insemination (TAI) is a very important tool for reproductive programs in small ruminants, providing synchronized inseminations and more efficient use of superior males. The objectives this study were to investigate the viability different breeding protocols under sub-optimal conditions out season, compare outcome TAI sheep with two semen extenders. Ewes (n=575) subjected estrou...

Semen, collected from boars of proven fertility, was concentrated by centrifuging the strained ejaculate at 400 g for 15 min at 20\s=deg\C and gently resuspending the sperm pellet in one-fifth the original volume of seminal plasma. The concentrated semen was then diluted 1:6 either in a chosen wash medium (see Table 1 for composition of media) and used immediately, or in a milk diluent (MFP), c...

Artificial insemination using sexing semen is expected to produce calves with the desired sex. One sperm method percoll density gradient centrifugation method. This study aimed determine changes and relationship between motility acrosome reaction after process centrifugation. The material used was of ±5 years old Ongole crossbred bull a bodyweight ±700 kg as many three bulls mass 2+ individual ...

Turkey sperm plasma membranes contain high levels of polyunsaturated fatty acids that are susceptible to lipid peroxidation during in vitro storage at 4 degrees C. Herein we assessed the degree of lipid peroxidation and fertility potential of semen liquid-stored for 24 h with the antioxidant vitamin E. Semen was collected weekly from 44 males and pooled as pairs (total = 22); the individuals in...

Semen from the first 15mL of the ejaculate (P1) obtained from two boars (30mL) was diluted in glycineegg yolk extender, cooled at 5°C in a special container and rediluted in standard doses of 3x10 mobile spermatozoa after 12h of storage. Semen was also stored up to 24h after redilution. The physical characteristics of the semen were evaluated at different storage periods (fresh, 0h, 12h, redilu...

There has and will continue to be reproductive techniques available that have a positive impact upon the equine breeding industry. This review focuses on semen technologies that have been developed or are in the process of being developed. The use of fluorescent dyes and flow cytometry has provided the researcher and clinician with powerful tools to evaluate several sperm attributes. These proc...

Background: Sperm cryopreservation is an important tool for breed improvement, nonetheless, spermatozoids of rams are extremely sensitive to cryopreservation. Aims: The present research was to compare a liposome-based (OptiXcell: OX) diluent, a commercial TRIS-egg yolk (Optidyl: OP) and a citrate egg yolk-based (CY) diluent on ovine semen quality through the cr...