Freezing of boar spermatozoa includes the cryoprotectant glycerol, but renders low cryosurvival, owing to major changes in osmolarity during freezing/thawing. We hypothesize that aquaporins (AQPs) 7 and 9 adapt their membrane domain location to these osmotic challenges, thus maintaining sperm homeostasis. Western blotting (WB) and immunocytochemistry (ICC) at light and electron microscope level...

In efforts to prepare more potent and sensitive viral serological antigens, several aspects of the production of antigens from infected cell cultures were studied. Antigens derived from whole, infected culture material and from the cellular and fluid phases were compared. Freezing and thawing, sonication, and alkaline buffer extraction were compared for effectiveness in releasing antigen from h...

[1] Much progress has been made in methane modeling for the Arctic. However, there is still large uncertainty in emissions estimates due to the spatial variability in water table depth resulting from complex topographic gradients, and due to variations in methane production and oxidation due to complex freezing and thawing processes. Here we extended an extant methane emission module within a b...

bstract: Air and surface freezing and thawing indexes and prediction of round temperatures from air temperatures were investigated. A new method for pplying n factors on a daily basis to capture localized temperature extremes is resented and compared to conventional seasonal n-factor analysis. Measured air emperatures from five locations and measured air and surface temperatures from ne locatio...

The microbial process of methane (CH4) production during the back-freezing of permafrost soils in autumn and the future fate of produced CH4 in the thawing phase of the following spring are not well understood. Long-term CH4 flux studies in the Lena Delta (Siberia) indicate that back-stored CH4 adds to the emission of newly-produced CH4 at the beginning of the vegetation period. Further field a...

Spermatogonial stem cells provide the foundation for continued adult spermatogenesis and their manipulation can facilitate assisted reproductive technologies or the development of transgenic animals. Because the pig is an important agricultural and biomedical research animal, the development of practical application techniques to manipulate the pig Spermatogonial stem cell is needed. The abilit...

background: nitric oxide (no) that plays important role in all sexual activities of animals is made from the amino acid l-arginine by the enzymatic action of no synthase (nos). no makes a band with sulfur-iron complexes, but due to production of steroid sexual hormones related to the enzymes involved in this complex, no can change the activity of these enzymes. no affects many cells including v...

The Qinghai–Tibet Plateau (QTP) is heavily affected by climate change and has been undergoing serious permafrost degradation due to global warming. Synthetic aperture radar interferometry (InSAR) has been a significant tool for mapping surface features or measuring physical parameters, such as soil moisture, active layer thickness, that can be used for permafrost modelling. This study analyzed ...

The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17°C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spermatozoa included: motility, normal apical ridg...

Understanding the processes of postmortem change in biologic systems is important to the forensic sciences. Previous experimental studies of postmortem change in animals under field conditions made use of animal carcasses that had been incidentally exposed to the effects of freezing and thawing or mechanical damage, or both, and were limited to gross observations. The current study was designed...